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. 2021 Aug;16(8):e2000575.
doi: 10.1002/biot.202000575. Epub 2021 Jun 19.

Recombineering facilitates the discovery of natural product biosynthetic pathways in Pseudomonas parafulva

Wentao Zheng  1 , Xue Wang  1 , Yuwei Chen  2 , Yachao Dong  2 , Diao Zhou  2 , Ruxin Liu  1 , Haibo Zhou  1 , Xiaoying Bian  1 , Hailong Wang  1 , Qiang Tu  1 , Vinothkannan Ravichandran  1 , Youming Zhang  1 , Aiying Li  1 , Jun Fu  1 , Jia Yin  2
Affiliations

Recombineering facilitates the discovery of natural product biosynthetic pathways in Pseudomonas parafulva

Wentao Zheng et al. Biotechnol J. 2021 Aug.

Abstract

Microbial natural products among other functions they play a vital role in the disease prevention in humans, animals and plants. Pseudomonas parafulva CRS01-1 is a broad-spectrum antagonistic bacterium present in plants. However, no natural products have been isolated from this strain till date. Corresponding biosynthetic gene clusters to natural products is an effective method for bioprospecting, for which, genome manipulation tools are essential. We previously developed Pseudomonas-specific phage-derived homologous recombination systems for genetic engineering in four Pseudomonas species. Herein, we report the application of these recombineering systems in Pseudomonas parafulva CRS01-1, along with structural elucidation and bioactivity evaluation of natural products. The Pseudomonas recombineering toolbox established before in different four species is efficient for genome mining and bioactive metabolite discovery from more distant species.

Keywords: Pseudomonas parafulva, recombineering; biosynthetic gene clusters, genome mining.

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References

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