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. 2021 Jan;1(1):e20.
doi: 10.1002/cpz1.20.

Growth and Maintenance of Escherichia coli Laboratory Strains

Amie R Tuttle  1 , Nicholas D Trahan  1 , Mike S Son  1
Affiliations

Growth and Maintenance of Escherichia coli Laboratory Strains

Amie R Tuttle et al. Curr Protoc. 2021 Jan.

Erratum in

Abstract

Escherichia coli is a Gram-negative bacterium, commonly used in both teaching and research laboratories. This article includes protocols for the growth and maintenance of E. coli in any teaching- or research-associated laboratory. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Growth of E. coli from frozen stocks Basic Protocol 2: Growth of E. coli in liquid media Basic Protocol 3: Enumeration of E. coli on solid media Basic Protocol 4: Storage of E. coli frozen stocks in glycerol Basic Protocol 5: Storage of E. coli in agar stabs Basic Protocol 6: Growth curve of E. coli liquid culture.

Keywords: Escherichia coli; growth; maintenance.

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