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. 2021 Jan;1(1):e18.
doi: 10.1002/cpz1.18.

Derivation of Mesenchymal Stromal Cells from Ovine Umbilical Cord Wharton's Jelly

Irene Carreras-Sánchez  1   2 , Alba López-Fernández  1   2 , Raquel Rojas-Márquez  1   2 , Roberto Vélez  2 , Màrius Aguirre  2 , Joaquim Vives  1   2   3
Affiliations

Derivation of Mesenchymal Stromal Cells from Ovine Umbilical Cord Wharton's Jelly

Irene Carreras-Sánchez et al. Curr Protoc. 2021 Jan.

Erratum in

Abstract

The methods described herein allow for the isolation and expansion of fibroblastic-like ovine Wharton's jelly-derived mesenchymal stromal cells (oWJ-MSC) that, similarly to their human counterparts, adhere to standard plastic surfaces in culture; show a mesenchymal profile for specific surface antigens (i.e., positive for CD44 and CD166); and lack expression of endothelial (CD31) and hematopoietic (CD45) markers as well as major histocompatibility complex (MHC) class-II. Homogeneous cell cultures result from a two-phase bioprocess design that starts with the isolation of mesenchymal stromal cells (MSC) from the Wharton's jelly of ovine umbilical cords up to a first step of cryopreservation. The second phase allows for further expansion of ovine WJ-MSC up to sufficient numbers for further studies. Overall, this methodology encompasses a 2-week bioprocess design that encompasses two cell culture passages ensuring sufficient cells for the generation of a Master Cell Bank. Further thawing and scale expansion results in large quantities of oWJ-MSC that can be readily used in proof of efficacy and safety studies in the preclinical development stage of the development of cell-based medicines. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Isolation and expansion of ovine mesenchymal stromal cells from Wharton's jelly of the umbilical cord Basic Protocol 2: Characterization of ovine mesenchymal stromal cells Basic Protocol 3: Growth profile determination of ovine mesenchymal stromal cells from Wharton's jelly.

Keywords: Wharton's jelly; derivation; multipotent mesenchymal stromal cells; non-clinical development; preclinical animal model; regenerative medicine; sheep; stem cell culture; translational medicine; umbilical cord.

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References

Literature Cited

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