Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021:2241:183-191.
doi: 10.1007/978-1-0716-1095-4_15.

Measurement of Eosinophil Kinetics In Vivo

Neda Farahi  1 , Daniel Gillett  2 , Chrystalla Loutsios  3 , A Michael Peters  4 , Charlotte Summers  3 , Edwin R Chilvers  5
Affiliations

Measurement of Eosinophil Kinetics In Vivo

Neda Farahi et al. Methods Mol Biol. 2021.

Abstract

Radiolabeled leukocyte scans are used in nuclear medicine to detect sites of infection and inflammation. We have previously demonstrated the use of clinical grade immunomagnetic beads to isolate autologous eosinophils and image their distribution in healthy volunteers. Here we describe the use of radiolabeled eosinophils coupled to single-photon emission computed tomography (SPECT) to quantify eosinophil uptake in the lungs of healthy volunteers, patients with asthma, and patients with focal eosinophilic inflammation.

Keywords: Cell trafficking; Eosinophils; Granulocytes; Imaging; Radiolabeling; SPECT.

PubMed Disclaimer

References

    1. Simon HU, Rothenberg ME, Bochner BB et al (2010) Refining the definition of hypereosinophilic syndrome. J Allergy Clin Immunol 126:45–49 - DOI
    1. Haldar P, Brightling CE, Hargadon B et al (2009) Mepolizumab and exacerbations of refractory eosinophilic asthma. N Engl J Med 360:973–984 - DOI
    1. Nair P, Pizzichini MM, Kjarsgaard M et al (2009) Mepolizumab for prednisone-dependent asthma with sputum eosinophilia. N Engl J Med 360:985–993 - DOI
    1. Furuta GT (2011) Eosinophilic esophagitis: update on clinicopathological manifestations and pathophysiology. Curr Opin Gastroenterol 27:383–388 - DOI
    1. Farahi N, Singh NR, Heard S et al (2012) Use of 111-Indium-labelled autologous eosinophils to establish the in vivo kinetics of human eosinophils in healthy subjects. Blood 120:4068–4071 - DOI

Publication types

MeSH terms

LinkOut - more resources