Transcription Factors as Evolvable Biosensors

doi:10.2116/analsci.20SCR12

Review

. 2021 May 10;37(5):699-703.

doi: 10.2116/analsci.20SCR12. Epub 2021 Jan 22.

Transcription Factors as Evolvable Biosensors

Daisuke Umeno¹, Yuki Kimura², Shigeko Kawai-Noma²

Affiliations

¹ Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi, Inage, Chiba, 263-8522, Japan. umeno@faculty.chiba-u.jp.
² Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi, Inage, Chiba, 263-8522, Japan.

PMID: 33487602
DOI: 10.2116/analsci.20SCR12

Free article

Review

Transcription Factors as Evolvable Biosensors

Daisuke Umeno et al. Anal Sci. 2021.

Free article

. 2021 May 10;37(5):699-703.

doi: 10.2116/analsci.20SCR12. Epub 2021 Jan 22.

Authors

Daisuke Umeno¹, Yuki Kimura², Shigeko Kawai-Noma²

Affiliations

¹ Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi, Inage, Chiba, 263-8522, Japan. umeno@faculty.chiba-u.jp.
² Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi, Inage, Chiba, 263-8522, Japan.

PMID: 33487602
DOI: 10.2116/analsci.20SCR12

Abstract

One of the most prominent features of genetically encoded biosensors (GEBs) is their evolvability-the ability to invent new sensory functions using mutations. Among the GEBs, the transcription factor-based biosensors (TF-biosensors) is the focus of this review. We also discuss how this class of sensors can be highly evolvable and how we can exploit it. With an established platform for directed evolution, researchers can create, or evolve, new TF-biosensors. Directed evolution experiments have revealed the TF-biosensors' evolvability, which is based partially on their characteristic physicochemical properties.

Keywords: Genetic switches; biosensors.

PubMed Disclaimer

Cited by

Microbial Transcription Factor-Based Biosensors: Innovations from Design to Applications in Synthetic Biology.
Song K, Ji H, Lee J, Yoon Y. Song K, et al. Biosensors (Basel). 2025 Mar 31;15(4):221. doi: 10.3390/bios15040221. Biosensors (Basel). 2025. PMID: 40277535 Free PMC article. Review.
Transcription factor-based biosensors for screening and dynamic regulation.
Tellechea-Luzardo J, Stiebritz MT, Carbonell P. Tellechea-Luzardo J, et al. Front Bioeng Biotechnol. 2023 Feb 6;11:1118702. doi: 10.3389/fbioe.2023.1118702. eCollection 2023. Front Bioeng Biotechnol. 2023. PMID: 36814719 Free PMC article. Review.
Biological Switches: Past and Future Milestones of Transcription Factor-Based Biosensors.
De Paepe B, De Mey M. De Paepe B, et al. ACS Synth Biol. 2025 Jan 17;14(1):72-86. doi: 10.1021/acssynbio.4c00689. Epub 2024 Dec 22. ACS Synth Biol. 2025. PMID: 39709556 Review.
Engineering of Synthetic Transcriptional Switches in Yeast.
Tominaga M, Kondo A, Ishii J. Tominaga M, et al. Life (Basel). 2022 Apr 8;12(4):557. doi: 10.3390/life12040557. Life (Basel). 2022. PMID: 35455048 Free PMC article. Review.

References

1. N. Lobsiger and W. J. Stark, Anal. Sci., 2019, 35, 839. - PubMed - DOI
1. H. Shiku, Anal. Sci., 2019, 35, 29. - PubMed - DOI
1. E. C. Greenwald, S. Mehta, and J. Zhang, Chem. Rev., 2018, 118, 11707. - PubMed - PMC - DOI
1. E. A. Rodriguez, R. E. Campbell, J. Y. Lin, M. Z. Lin, A. Miyawaki, A. E. Palmer, X. Shu, J. Zhang, and R. Y. Tsien, Trends Biochem. Sci., 2017, 42, 111. - PubMed - DOI
1. V. Marx, Nat. Methods, 2017, 14, 949. - PubMed - DOI

Publication types

Actions

MeSH terms

Actions
Actions
Actions

Substances

Actions

LinkOut - more resources

Full Text Sources
- J-STAGE, Japan Science and Technology Information Aggregator, Electronic
Other Literature Sources
- scite Smart Citations
Miscellaneous
- NCI CPTAC Assay Portal

[1] N. Lobsiger and W. J. Stark, Anal. Sci., 2019, 35, 839. - PubMed - DOI

[2] N. Lobsiger and W. J. Stark, Anal. Sci., 2019, 35, 839. - PubMed - DOI

[3] H. Shiku, Anal. Sci., 2019, 35, 29. - PubMed - DOI

[4] H. Shiku, Anal. Sci., 2019, 35, 29. - PubMed - DOI

[5] E. C. Greenwald, S. Mehta, and J. Zhang, Chem. Rev., 2018, 118, 11707. - PubMed - PMC - DOI

[6] E. C. Greenwald, S. Mehta, and J. Zhang, Chem. Rev., 2018, 118, 11707. - PubMed - PMC - DOI

[7] E. A. Rodriguez, R. E. Campbell, J. Y. Lin, M. Z. Lin, A. Miyawaki, A. E. Palmer, X. Shu, J. Zhang, and R. Y. Tsien, Trends Biochem. Sci., 2017, 42, 111. - PubMed - DOI

[8] E. A. Rodriguez, R. E. Campbell, J. Y. Lin, M. Z. Lin, A. Miyawaki, A. E. Palmer, X. Shu, J. Zhang, and R. Y. Tsien, Trends Biochem. Sci., 2017, 42, 111. - PubMed - DOI

[9] V. Marx, Nat. Methods, 2017, 14, 949. - PubMed - DOI

[10] V. Marx, Nat. Methods, 2017, 14, 949. - PubMed - DOI

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Transcription Factors as Evolvable Biosensors

Affiliations

Transcription Factors as Evolvable Biosensors

Authors

Affiliations

Abstract

Similar articles

Cited by

References

Publication types

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Miscellaneous