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. 2021 Feb;11(2):45.
doi: 10.1007/s13205-020-02587-6. Epub 2021 Jan 11.

Characterization of high-temperature stress-tolerant tomato (Solanum lycopersicum L.) genotypes by biochemical analysis and expression profiling of heat-responsive genes

Suhas Gorakh Karkute  1 , Waquar Akhter Ansari  1 , Achuit Kumar Singh  1 , Prabhakar Mohan Singh  1 , Nagendra Rai  1 , Anant Bahadur  1 , Jagdish Singh  1
Affiliations

Characterization of high-temperature stress-tolerant tomato (Solanum lycopersicum L.) genotypes by biochemical analysis and expression profiling of heat-responsive genes

Suhas Gorakh Karkute et al. 3 Biotech. 2021 Feb.

Abstract

High-temperature stress severely impacts both yield and quality of tomato fruits, and therefore, it is required to develop stress-tolerant cultivars. In the present study, two tomato genotypes, H88-78-1 and CLN-1621, identified through preliminary phenotypic screening were characterized by analysis of molecular, physiological, and biochemical traits in comparison with a susceptible genotype Punjab Chhuhara. Phenotypic stress tolerance of both the genotypes was validated at biochemical level as they showed higher amount of relative water content, photosynthetic pigments, free cellular proline, and antioxidant molecules while less amount of H2O2 and electrolyte leakage. Expression analysis of 67 genes including heat shock factors, heat shock proteins, and other stress-responsive genes showed significant up-regulation of many of the genes such as 17.4 kDa class III heat shock protein, HSF A-4a, HSF30, HSF B-2a, HSF24, HSF B-3 like, 18.1 kDa class I HSP like, and HSP17.4 in H88-78-1 and CLN-1621 after exposure to high-temperature stress. These candidate genes can be transferred to cultivated varieties by developing gene-based markers and marker-assisted breeding. This confirms the rapid response of these genotypes to high-temperature stress. All these traits are characteristics of a stress-tolerance and establish them as candidate high-temperature stress-tolerant genotypes that can be effectively utilized in stress tolerance improvement programs.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-020-02587-6.

Keywords: Gene expression; Heat shock proteins; High-temperature stress; Tolerance; Tomato.

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Conflict of interest statement

Conflict of interestThe authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Physical map of high-temperature stress-responsive genes on 12 chromosomes of tomato. Numerical values show size in mega base pairs
Fig. 2
Fig. 2
Heatmap and hierarchical clustering showing expression pattern of 67 high-temperature stress-responsive genes in three tomato genotypes at different time intervals (0 h, 16 h, 32 h, and 48 h) of stress treatment. PBC: Punjab Chuhara, CLN: CLN1621, H88: H88-78-1
See this image and copyright information in PMC

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