Development of a protocol for detection of SARS-CoV-2 in sputum and endotracheal aspirates using Cepheid Xpert Xpress SARS-CoV-2

doi:10.1099/acmi.0.000176

. 2020 Nov 12;2(12):acmi000176.

doi: 10.1099/acmi.0.000176. eCollection 2020.

Development of a protocol for detection of SARS-CoV-2 in sputum and endotracheal aspirates using Cepheid Xpert Xpress SARS-CoV-2

Michael Malczynski¹, Saba Rezaeian¹, Javier Rios¹, Laura Dirnberger¹, Wanda Polanco¹, Teresa Zembower^{1

2

3}, Chao Qi^{1

3}

Affiliations

¹ Clinical Microbiology Laboratory, Department of Pathology, Northwestern Memorial Hospital, Chicago, IL, USA.
² Division of Infectious Diseases, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
³ Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.

PMID: 33490871
PMCID: PMC7818244
DOI: 10.1099/acmi.0.000176

Development of a protocol for detection of SARS-CoV-2 in sputum and endotracheal aspirates using Cepheid Xpert Xpress SARS-CoV-2

Michael Malczynski et al. Access Microbiol. 2020.

. 2020 Nov 12;2(12):acmi000176.

doi: 10.1099/acmi.0.000176. eCollection 2020.

Authors

Michael Malczynski¹, Saba Rezaeian¹, Javier Rios¹, Laura Dirnberger¹, Wanda Polanco¹, Teresa Zembower^{1

2

3}, Chao Qi^{1

3}

Affiliations

¹ Clinical Microbiology Laboratory, Department of Pathology, Northwestern Memorial Hospital, Chicago, IL, USA.
² Division of Infectious Diseases, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
³ Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.

PMID: 33490871
PMCID: PMC7818244
DOI: 10.1099/acmi.0.000176

Abstract

Sputum and endotracheal aspirates (ETs) are not among the vendor-approved specimens for the Cepheid Xpert SARS-CoV-2 assay. However, they are the common lower respiratory tract specimens submitted for laboratory diagnosis. Testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in lower respiratory tract samples is required for the discharge of patients from coronavirus disease (COVID) units at some institutions. We developed a protocol used for testing unliquified viscous sputum or tracheal aspirate with the Cepheid Xpert SARS-CoV-2 assay.

Keywords: sputum; Cepheid Xpert Xpress; SARS-CoV-2; endotracheal aspirate; rapid detection.

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Conflict of interest statement

The authors declare that there are no conflicts of interest.

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References

1. Wölfel R, Corman VM, Guggemos W, Seilmaier M, Zange S, et al. Virological assessment of hospitalized patients with COVID-2019. Nature. 2020;581:465–469. doi: 10.1038/s41586-020-2196-x. - DOI - PubMed
1. Lan L, Xu D, Ye G, Xia C, Wang S, et al. Positive RT-PCR test results in patients recovered from COVID-19. JAMA. 2020;323:1502. doi: 10.1001/jama.2020.2783. - DOI - PMC - PubMed
1. Cepheid Cepheid Xpert Xpress SARS-CoV-2 Instructions for Use. April 2020(Rev C) 3260
1. FDA Statistical guidance on reporting results from studies evaluating diagnostic tests. 2007.

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[1] Wölfel R, Corman VM, Guggemos W, Seilmaier M, Zange S, et al. Virological assessment of hospitalized patients with COVID-2019. Nature. 2020;581:465–469. doi: 10.1038/s41586-020-2196-x. - DOI - PubMed

[2] Wölfel R, Corman VM, Guggemos W, Seilmaier M, Zange S, et al. Virological assessment of hospitalized patients with COVID-2019. Nature. 2020;581:465–469. doi: 10.1038/s41586-020-2196-x. - DOI - PubMed

[3] Lan L, Xu D, Ye G, Xia C, Wang S, et al. Positive RT-PCR test results in patients recovered from COVID-19. JAMA. 2020;323:1502. doi: 10.1001/jama.2020.2783. - DOI - PMC - PubMed

[4] Lan L, Xu D, Ye G, Xia C, Wang S, et al. Positive RT-PCR test results in patients recovered from COVID-19. JAMA. 2020;323:1502. doi: 10.1001/jama.2020.2783. - DOI - PMC - PubMed

[5] Cepheid Cepheid Xpert Xpress SARS-CoV-2 Instructions for Use. April 2020(Rev C) 3260

[6] Cepheid Cepheid Xpert Xpress SARS-CoV-2 Instructions for Use. April 2020(Rev C) 3260

[7] FDA Statistical guidance on reporting results from studies evaluating diagnostic tests. 2007.

[8] FDA Statistical guidance on reporting results from studies evaluating diagnostic tests. 2007.

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Development of a protocol for detection of SARS-CoV-2 in sputum and endotracheal aspirates using Cepheid Xpert Xpress SARS-CoV-2

Affiliations

Development of a protocol for detection of SARS-CoV-2 in sputum and endotracheal aspirates using Cepheid Xpert Xpress SARS-CoV-2

Authors

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Abstract

Conflict of interest statement

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