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. 2020 Nov 12;2(12):acmi000176.
doi: 10.1099/acmi.0.000176. eCollection 2020.

Development of a protocol for detection of SARS-CoV-2 in sputum and endotracheal aspirates using Cepheid Xpert Xpress SARS-CoV-2

Michael Malczynski  1 , Saba Rezaeian  1 , Javier Rios  1 , Laura Dirnberger  1 , Wanda Polanco  1 , Teresa Zembower  1   2   3 , Chao Qi  1   3
Affiliations

Development of a protocol for detection of SARS-CoV-2 in sputum and endotracheal aspirates using Cepheid Xpert Xpress SARS-CoV-2

Michael Malczynski et al. Access Microbiol. .

Abstract

Sputum and endotracheal aspirates (ETs) are not among the vendor-approved specimens for the Cepheid Xpert SARS-CoV-2 assay. However, they are the common lower respiratory tract specimens submitted for laboratory diagnosis. Testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in lower respiratory tract samples is required for the discharge of patients from coronavirus disease (COVID) units at some institutions. We developed a protocol used for testing unliquified viscous sputum or tracheal aspirate with the Cepheid Xpert SARS-CoV-2 assay.

Keywords: sputum; Cepheid Xpert Xpress; SARS-CoV-2; endotracheal aspirate; rapid detection.

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Conflict of interest statement

The authors declare that there are no conflicts of interest.

References

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