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. 2021 Jan 12;2(1):100267.
doi: 10.1016/j.xpro.2020.100267. eCollection 2021 Mar 19.

Isolation of tumor-resident CD8+ T cells from human lung tumors

Stéphanie Corgnac  1 , Yann Lecluse  2 , Fathia Mami-Chouaib  1
Affiliations

Isolation of tumor-resident CD8+ T cells from human lung tumors

Stéphanie Corgnac et al. STAR Protoc. .

Abstract

CD103+CD8+ tumor-resident memory T cells (TRM) are important components of anti-tumor immunity. However, their role in response to cancer immunotherapy is not fully understood. The protocol describes how to isolate CD8+ T cells and autologous tumor cells from human lung tumors to study the functional activities of CD8+ T cells. Tumors are heterogeneous in terms of the quantity and quality of immune cell types, so the yield of TRM cells depends on the features of the tumor. For complete details on the use and execution of this protocol, please refer to Corgnac et al. (2020).

Keywords: Cancer; Cell isolation; Flow cytometry/mass cytometry; Immunology.

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Conflict of interest statement

The authors declare no competing interests.

Figures

None
Graphical abstract
Figure 1
Figure 1
Gating strategy for analyses of tumor-infiltrating CD8+CD103+ TRM cells by flow cytometry
Figure 2
Figure 2
Gating strategy for sorting CD8+CD103+ and CD8+KLRG1+ cells by FACS
Figure 3
Figure 3
Purity of sorted CD8+ TRM cells post-FACS
See this image and copyright information in PMC

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