Genomic Diversity of Burkholderia pseudomallei Isolates, Colombia

Abstract

We report an analysis of the genomic diversity of isolates of Burkholderia pseudomallei, the cause of melioidosis, recovered in Colombia from routine surveillance during 2016-2017. B. pseudomallei appears genetically diverse, suggesting it is well established and has spread across the region.

Keywords: Burkholderia pseudomallei; Colombia; bacteria; genomic diversity; melioidosis; molecular epidemiology; multilocus sequence typing; phylogeography; whole-genome sequencing.

Figure

Dendrogram used for characterization of Burkholderia pseudomallei genomes from Colombia compared with reference genomes. Tree was generated in MEGA7 software (http://www.megasoftware.net) from results of maximum-parsimony phylogenetic analysis of core single-nucleotide polymorphisms conducted by using Parsnp, a component of the Harvest 1.3 software suite (https://github.com). Bold branches indicate the subclade containing the examples from Colombia along with reference genomes that group with them. Isolates from Colombia also include the department where they originated. Scale bar indicates number of substitutions per single nucleotide polymorphism.