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. 2021 Jan 20;9(2):209.
doi: 10.3390/microorganisms9020209.

Multicenter Evaluation of an ELISA for the Detection of Cryptosporidium spp. Antigen in Clinical Human Stool Samples

Romy Razakandrainibe  1   2 , Célia Mérat  3 , Nathalie Kapel  4 , Marc Sautour  5 , Karine Guyot  6 , Gilles Gargala  1   2 , Jean-Jacques Ballet  1 , Patrice Le Pape  3 ; French Cryptosporidiosis Network; Frédéric Dalle  5   7 , Loïc Favennec  1   2
Collaborators, Affiliations

Multicenter Evaluation of an ELISA for the Detection of Cryptosporidium spp. Antigen in Clinical Human Stool Samples

Romy Razakandrainibe et al. Microorganisms. .

Abstract

Human cryptosporidiosis remains underdiagnosed, and rapid/accurate diagnosis is of clinical importance. Diagnosis of the Cryptosporidium oocyst in stool samples by conventional microscopy is labor-intensive, time-consuming, and requires skillful experience. Thus, we aimed to evaluate the usefulness of a coproantigen enzyme-linked immunosorbent assay (ELISA) test in detecting Cryptosporidium spp. from fecal specimens. For this aim, we evaluated the performances of a commercial ELISA (CoproELISA Cryptosporidium kit, Savyon Diagnostics, Israel) for the detection of Cryptosporidium spp. in random clinical stool samples through a multicenter study. The sensitivity and specificity for coproantigen ELISA were 98.86% and 94.32%, respectively. The coproantigen ELISA results indicate that the simple, rapid, reliable, and standardized immunoassay test is sensitive and specific for routine diagnosis, and may be useful for large-scale epidemiological studies of cryptosporidiosis.

Keywords: Cryptosporidium spp.; coproantigen ELISA; cryptosporidiosis; diagnosis; large-scale epidemiology.

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Conflict of interest statement

The authors declare no conflict of interest.

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