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Review
. 2021 Jan 23;22(3):1121.
doi: 10.3390/ijms22031121.

Assessing Kidney Graft Viability and Its Cells Metabolism during Machine Perfusion

Maria Irene Bellini  1   2 , Francesco Tortorici  3   4 , Maria Ida Amabile  2 , Vito D'Andrea  2
Affiliations
Review

Assessing Kidney Graft Viability and Its Cells Metabolism during Machine Perfusion

Maria Irene Bellini et al. Int J Mol Sci. .

Abstract

Kidney transplantation is the golden treatment for end-stage renal disease. Static cold storage is currently considered the standard method of preservation, but dynamic techniques, such as machine perfusion (MP), have been shown to improve graft function, especially in kidneys donated by extended criteria donors and donation after circulatory death. With poor organ quality being a major reason for kidneys not being transplanted, an accurate, objective and reliable quality assessment during preservation could add value and support to clinicians' decisions. MPs are emerging technologies with the potential to assess kidney graft viability and quality, both in the hypothermic and normothermic scenarios. The aim of this review is to summarize current tools for graft viability assessment using MP prior to implantation in relation to the ischemic damage.

Keywords: ischemic reperfusion injury; kidney transplantation; organ preservation.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Cell metabolism. Glycolysis produces pyruvate and NADH in the cytoplasm. In the absence of oxygen, pyruvate is reduced to lactate (or to another fermentation product as ethanol), that is eliminated and NAD+ is reutilized to continue glycolysis. In the presence of oxygen, pyruvate is decarboxylised and linked to A coenzyme (CoA), to produce NADH. NADH donates its electrons to mitochondria, until the final chain element, that is the oxygen. The energy released by NADH during electron transports is used to produce ATP.
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References

    1. Wolfe R.A., Ashby V.B., Milford E.L., Ojo A.O., Ettenger R.E., Agodoa L.Y., Held P.J., Port F.K. Comparison of Mortality in All Patients on Dialysis, Patients on Dialysis Awaiting Transplantation, and Recipients of a First Cadaveric Transplant. N. Engl. J. Med. 1999;341:1725–1730. doi: 10.1056/NEJM199912023412303. - DOI - PubMed
    1. Kamińska D., Kościelska-Kasprzak K., Chudoba P., Hałoń A., Mazanowska O., Gomółkiewicz A., Dzięgiel P., Drulis-Fajdasz D., Myszka M., Lepiesza A., et al. The influence of warm ischemia elimination on kidney injury during transplantation–clinical and molecular study. Sci. Rep. 2016;6:36118. doi: 10.1038/srep36118. - DOI - PMC - PubMed
    1. Peters-Sengers H., Houtzager J.H.E., Heemskerk M.B.A., Idu M.M., Minnee R.C., Klaasen R.W., Joor S.E., Hagenaars J.A.M., Rebers P.M., Van Der Heide J.J.H., et al. DCD donor hemodynamics as predictor of outcome after kidney transplantation. Arab. Archaeol. Epigr. 2018;18:1966–1976. doi: 10.1111/ajt.14676. - DOI - PubMed
    1. Port F.K., Bragg-Gresham J.L., Metzger R.A., Dykstra D.M., Gillespie B.W., Young E.W., Delmonico F.L., Wynn J.J., Merion R.M., Wolfe R.A., et al. Donor characteristics associated with reduced graft survival: An approach to expanding the pool of kidney donors1. Transplantation. 2002;74:1281–1286. doi: 10.1097/00007890-200211150-00014. - DOI - PubMed
    1. Lam N.N., Boyne D.J., Quinn R.R., Austin P.C., Hemmelgarn B.R., Campbell P., Knoll G.A., Tibbles L.A., Yilmaz S., Quan H., et al. Mortality and Morbidity in Kidney Transplant Recipients with a Failing Graft: A Matched Cohort Study. Can. J. Kidney Health Dis. 2020;7:2054358120908677. doi: 10.1177/2054358120908677. - DOI - PMC - PubMed

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