The porcine respiratory microbiome: recent insights and future challenges

Abstract

Understanding the structure of the respiratory microbiome and its complex interactions with opportunistic pathogenic bacteria has become a topic of great scientific and economic interest in livestock production, given the severe consequences of respiratory disease on animal health and welfare. The present review focuses on the microbial structures of the porcine upper and lower airways, and the factors that influence microbiome development and onset of respiratory disease. Following a literature search on PubMed and Scopus, 21 articles were selected based on defined exclusion criteria (20 studies performed by 16S rRNA gene sequencing and one by shotgun metagenomics). Analysis of the selected literature indicated that the microbial structure of the upper respiratory tract undergoes a remarkable evolution after birth and tends to stabilise around weaning. Antimicrobial treatment, gaseous ammonia concentration, diet and floor type are amongst the recognized environmental factors influencing microbiome structure. The predominant phyla of the upper respiratory tract are Proteobacteria and Firmicutes with significant differences at the genus level between the nasal and the oropharyngeal cavity. Only five studies investigated the lower respiratory tract and their results diverged in relation to the relative abundance of these two phyla and even more in the composition of the lung microbiome at the genus level, likely because of methodological differences. Reduced diversity and imbalanced microbial composition are associated with an increased risk of respiratory disease. However, most studies presented methodological pitfalls concerning specimen collection, sequencing target and depth, and lack of quality control. Standardization of sampling and sequencing procedures would contribute to a better understanding of the structure of the microbiota inhabiting the lower respiratory tract and its relationship with pig health and disease.

Keywords: Disease; Health; Microbiota; Pig; Porcine respiratory disease complex; Respiratory tract.

Fig. 1

Flowchart of the literature search and studies selection

Fig. 2

Bacterial topography and factors shaping the porcine respiratory microbiome. The porcine respiratory microbiome is divided into the URT (upper pie chart) and the LRT (lower pie chart), which are mainly colonized by members of the Proteobacteria, Firmicutes, and Bacteroidetes. Under physiological conditions, the respiratory tract hosts a rich and diverse microbial community (left circle). Several factors, including respiratory disease onset, farm management practices and antimicrobial treatment, contribute to reducing the bacterial diversity in the imbalance state (right circle)