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. 2021 Feb;149(1):73-80.
doi: 10.1007/s10709-021-00114-w. Epub 2021 Jan 27.

Streptomycin and nalidixic acid elevate the spontaneous genome-wide mutation rate in Escherichia coli

Huseyin Ozgur Ozdemirel  1 , Dilara Ulusal  1 , Sibel Kucukyildirim Celik  2
Affiliations

Streptomycin and nalidixic acid elevate the spontaneous genome-wide mutation rate in Escherichia coli

Huseyin Ozgur Ozdemirel et al. Genetica. 2021 Feb.

Abstract

Since antibiotic resistance is a growing public health problem worldwide, it is important to understand how antibiotics and spontaneous mutations cooperate and shape the genome-wide mutation rate and spectrum. Here, we quantitatively evaluate genome-wide mutational profiles of Escherichia coli after long-term subinhibitory exposure to a broad-spectrum (streptomycin) and a narrow-spectrum antibiotic (nalidixic acid), using a mutation accumulation design combined with whole-genome resequencing of replicate lines as a mutagenicity test. We determined that, while the genome-wide mutation rate is slightly higher in the streptomycin-treated lines compared to the control lines, there is a significant increase in the nalidixic acid-treated lines. Our findings suggest that both broad and narrow-spectrum antibiotics may elevate the mutation rates in E. coli, but mechanisms of action may affect the consequence, thus contribute to accelerating the rate of adaptation and conferring antibiotic resistance.

Keywords: Antibiotic resistance; Mutagenicity test; Mutation accumulation; Mutation spectrum.

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References

    1. Boe L, Tolker-Nielsen T, Eeegholm KM, Spliid H, Vrang A (1994) Fluctuation analysis of mutations to nalidixic acid resistance in Escherichia coli. J Bacteriol 176:2781–2787. https://doi.org/10.1128/jb.176.10.2781-2787.1994 - DOI - PubMed - PMC
    1. Chu X-L, Zhang B-W, Zhang Q-G, Zhu B-R, Lin K, Zhang D-Y (2018) Temperature responses of mutation rate and mutational spectrum in an Escherichia coli strain and the correlation with metabolic rate. BMC Evol Biol 18:126. https://doi.org/10.1186/s12862-018-1252-8 - DOI - PubMed - PMC
    1. Davies J, Davies D (2010) Origins and evolution of antibiotic resistance. Microbiol Mol Biol Rev 74:417–433. https://doi.org/10.1128/MMBR.00016-10 - DOI - PubMed - PMC
    1. DePristo MBE et al (2011) A framework for variation discovery and genotyping using next-generation DNA sequencing data. Nat Genet 43:491–498. https://doi.org/10.1038/ng.806 - DOI - PubMed - PMC
    1. Didier J-P, Villet R, Huggler E, Lew DP, Hooper DC, Kelley WL, Vaudaux P (2011) Impact of ciprofloxacin exposure on Staphylococcus aureus genomic alterations linked with emergence of rifampin resistance. Antimicrob Agents Chemother 55:1946–1952. https://doi.org/10.1128/AAC.01407-10 - DOI - PubMed - PMC

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