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Review
. 2021 Mar;113(3):337-343.
doi: 10.1007/s12185-021-03085-y. Epub 2021 Jan 27.

Monitoring minimal/measurable residual disease in B-cell acute lymphoblastic leukemia by flow cytometry during targeted therapy

Zhiyu Liu  1 , Yang Li  2 , Ce Shi  3
Affiliations
Review

Monitoring minimal/measurable residual disease in B-cell acute lymphoblastic leukemia by flow cytometry during targeted therapy

Zhiyu Liu et al. Int J Hematol. 2021 Mar.

Abstract

B-cell acute lymphoblastic leukemia (B-ALL) is a hematologic malignancy of B-type lymphoid precursor cells. Minimal/measurable residual disease (MRD) is an important prognostic factor for B-ALL relapse. Traditional flow cytometry detection mainly relies on CD19-based gating strategies. However, relapse of CD19-negative B-ALL frequently occurs in patients who receive cellular and targeted therapy. This review will summarize the technical aspects of standard MRD assessment in B-ALL by flow cytometry, and then discuss the challenges of MRD strategies to deal with the scenario of CD19 negative or dim B-ALL relapse.

Keywords: Acute lymphoblastic leukemia; Minimal/measurable residual disease; Targeted therapy.

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