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. 2021 Jan 20;35(12):e351203.
doi: 10.1590/ACB351203. eCollection 2021.

Age-dependent histomorphometric evolution of the corpus spongiosum

Gino Pigatto Filho  1 , Marcelo Zeni  2 , Eduardo Felippe Melchioretto  2 , Gustavo Lenci Marques  2 , Thiago Hota  2 , Rogério de Fraga  3
Affiliations

Age-dependent histomorphometric evolution of the corpus spongiosum

Gino Pigatto Filho et al. Acta Cir Bras. .

Abstract

Purpose: To quantify the age-dependent changes in the relative volume of elastic fibers, collagen fibers and the proportion of collagen types I/III in the corpus spongiosum of rats.

Methods: Forty-eight rats, raised under similar conditions, were divided into four groups (G1 to G4) and underwent penectomy at the ages of 6, 9, 12 and 24 months, respectively. Histological sections from the middle segment of the penis were stained with Weigert's resorcin-fuchsin, Masson's trichrome and Picrosirius red, the volumetric density of elastic fibers, collagen fibers and the proportion of collagen types I and III in the corpus spongiosum were determined by stereological analysis.

Results: A reduction in the proportion of collagen I/III between the groups G3 and G4 (p < 0.048) was observed. In the volumetric analysis of elastic fiber, we observed a significant rise between the groups G2 and G3 (p < 0.03) and a reduction of the volume between the groups G3 and G4 (p < 0.01). However, there was no difference in the quantity of total collagen between the groups (p > 0.54).

Conclusions: Aging in rats did not change the quantity of total collagen but reduced the proportion of collagen types I/III and the volume of elastic fibers.

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Conflict of interest statement

Conflict of interest:none

Figures

Figure 1
Figure 1. Corpus spongiosum stained with (a) Picrosirius red staining method visualized under polarized light. The fibers stained in the specter between red and yellow are collagen type I and those in green are collagen type III. The purple line delimitates the area of CS. (b) Corpus spongiosum stained with Masson’s trichrome staining method. Blue areas indicated by the red arrow are collagen fibers. (c) Corpus spongiosum stained with Weigert’s resorcin-fuchsin method. The blue arrow indicates the border of the corpus spongiosum and green arrow indicates elastic fibers.
Figure 2
Figure 2. Graphs representing the correlation between months (ages) and (a) collagen type I/III, (b) total collagen and (c) elastic fibers.
See this image and copyright information in PMC

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