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. 2021 Jan 25;9(2):242.
doi: 10.3390/microorganisms9020242.

Genomic Characterization of Clinical Extensively Drug-Resistant Acinetobacter pittii Isolates

Peechanika Chopjitt  1 , Nuntiput Putthanachote  2 , Ratchadaporn Ungcharoen  1 , Rujirat Hatrongjit  3 , Parichart Boueroy  1 , Yukihiro Akeda  4   5 , Kazunori Tomono  4 , Shigeyuki Hamada  5 , Anusak Kerdsin  1
Affiliations

Genomic Characterization of Clinical Extensively Drug-Resistant Acinetobacter pittii Isolates

Peechanika Chopjitt et al. Microorganisms. .

Abstract

Carbapenem-resistant Acinetobacter pittii (CRAP) is a causative agent of nosocomial infections. This study aimed to characterize clinical isolates of CRAP from a tertiary hospital in Northeast Thailand. Six isolates were confirmed as extensively drug-resistant Acinetobacter pittii (XDRAP). The blaNDM-1 gene was detected in three isolates, whereas blaIMP-14 and blaIMP-1 were detected in the others. Multilocus sequence typing with the Pasteur scheme revealed ST220 in two isolates, ST744 in two isolates, and ST63 and ST396 for the remaining two isolates, respectively. Genomic characterization revealed that six XDRAP genes contained antimicrobial resistance genes: ST63 (A436) and ST396 (A1) contained 10 antimicrobial resistance genes, ST220 (A984 and A864) and ST744 (A56 and A273) contained 9 and 8 antimicrobial resistance genes, respectively. The single nucleotide polymorphism (SNP) phylogenetic tree revealed that the isolates A984 and A864 were closely related to A. pittii YB-45 (ST220) from China, while A436 was related to A. pittii WCHAP100020, also from China. A273 and A56 isolates (ST744) were clustered together; these isolates were closely related to strains 2014S07-126, AP43, and WCHAP005069, which were isolated from Taiwan and China. Strict implementation of infection control based upon the framework of epidemiological analyses is essential to prevent outbreaks and contain the spread of the pathogen. Continued surveillance and close monitoring with molecular epidemiological tools are needed.

Keywords: Acinetobacter pittii; Thailand; XDR; carbapenem-resistance; extensively drug-resistant; whole genome sequencing.

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Conflict of interest statement

The authors have no conflicts of interest in this study.

Figures

Figure 1
Figure 1
Minimum spanning tree of sequence types (ST) of 65 A. pittii, constructed with goeBURST. The seven CRAP isolates belonging to four STs are denoted as red circles.
Figure 2
Figure 2
Genomic characterization of antibiotic-resistant genes in carbapenem-resistant A. pittii. The present of antimicrobial resistance genes is represented in red box.
Figure 3
Figure 3
Whole-genome phylogeny analysis of A. pittii generated by CSI Phylogeny and visualized with interactive tree of life tool. The whole genome sequence of A. pittii in our studies is shown in yellow highlight and A. pittii-ST220-China as a reference genome is denote in red square box. Sequence type (STs) and β-lactamase genes are shown in each isolate. The filled symbols reveal the presentation of the genes, whereas unfilled symbols reveal their absence.
See this image and copyright information in PMC

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