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Review
. 2021 Jan;41(1):145-152.
doi: 10.5851/kosfa.2020.e78. Epub 2021 Jan 1.

Bioconversion Products of Whey by Lactic Acid Bacteria Exert Anti-Adipogenic Effect

Ji Soo Lee  1 , In Kyung Hyun  1 , Ji-Won Yoon  1 , Hye-Jin Seo  1 , Seok-Seong Kang  1
Affiliations
Review

Bioconversion Products of Whey by Lactic Acid Bacteria Exert Anti-Adipogenic Effect

Ji Soo Lee et al. Food Sci Anim Resour. 2021 Jan.

Abstract

Microbial bioconversion using lactic acid bacteria (LAB) provides several human health benefits. Although whey and whey-derived bioactive compounds can contribute to an improvement in human health, the potential anti-obesity effect of whey bioconversion by LAB has not been well studied. This study aimed to investigate whether bioconversion of whey by Pediococcus pentosaceus KI31 and Lactobacillus sakei KI36 (KI31-W and KI36-W, respectively) inhibits 3T3-L1 preadipocyte differentiation. Both KI31-W and KI36-W reduced intracellular lipid accumulation significantly, without decreasing 3T3-L1 preadipocyte proliferation. In addition, obesity-related transcription factor (peroxisome proliferator-activated receptor γ) and genes (adipocyte fatty acid-binding protein and lipoprotein lipase) were down-regulated significantly in 3T3-L1 cells in the presence of KI31-W and KI36-W. Collectively, these results suggest that bioconversion of whey by LAB exhibits anti-adipogenic activity and may be applied as a therapeutic agent for obesity.

Keywords: 3T3-L1 preadipocyte; bioconversion; lactic acid bacteria; obesity; whey.

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Conflict of interest statement

The authors declare no potential conflicts of interest.

Figures

Fig. 1.
Fig. 1.. Cell viability of 3T3-L1 adipocytes in the presence of KI 31-W and KI 36-W.
The cell viability was determined by MTT assay after 3T3-L1 cells were treated with KI 31-W or KI 36-W for 24 h. The results were obtained from three independent experiments.
Fig. 2.
Fig. 2.. Effect of KI 31-W and KI 36-W on lipid accumulation in 3T3-L1 adipocytes.
Determination of lipid content by Oil Red O staining in fully differentiated 3T3-L1 adipocytes treated with KI 31-W (A) or KI 36-W (B). Images represent microscopy of Oil Red O-stained cells. The results were obtained from three independent experiments. (C) lipid accumulation in 3T3-L1 cells in the presence of non-bioconverted whey was determined by Oil Red O staining. (D) protein profiles of non-bioconverted and bioconversion of whey were analyzed by SDS-PAGE. M, size marker.
Fig. 3.
Fig. 3.. Effect of KI 31-W and KI 36-W on the mRNA levels of adipogenesis- and lipogenesis-related genes in 3T3-L1 adipocytes.
The mRNA expression of PPARγ, aP2, and LPL in 3T3-L1 adipocytes in the presence of KI 31-W or KI 36-W were quantified by real-time RT-PCR analysis. The results were obtained from three independent experiments. PPARγ, peroxisome proliferator-activated receptor.
See this image and copyright information in PMC

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