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. 2021 Jan 28;43(1):2.
doi: 10.1186/s41021-021-00173-7.

Species diversity and molecular analysis of opportunistic Mycobacterium, Nocardia and Rhodococcus isolated from the hospital environment in a developing country, a potential resources for nosocomial infection

Marzieh Siavashifar  1 , Fatemeh Rezaei  1 , Tahereh Motallebirad  2 , Davood Azadi  3 , Abdorrahim Absalan  2 , Zahra Naserramezani  1 , Mohadeseh Golshani  1 , Morteza Jafarinia  4 , Kazem Ghaffari  2
Affiliations

Species diversity and molecular analysis of opportunistic Mycobacterium, Nocardia and Rhodococcus isolated from the hospital environment in a developing country, a potential resources for nosocomial infection

Marzieh Siavashifar et al. Genes Environ. .

Erratum in

Abstract

Background: Hospital environmental resources have a significant role in cross-transmission of opportunistic pathogens such as actinomycetes species to the patients. Actinomycetes have a remarkable capability to survive in adverse and harsh conditions of hospital environments; therefore, they are a threat to the health of patients. Due to this issue, we aimed to determine the frequency and diversity of actinomycetes species in hospital soil, water and dust by using a combination of conventional and molecular methods including the phenotypic and biochemical tests for preliminary identification and the PCR amplification of the specific region of the 16S rRNA, hsp65 gene and sequence analyses of 16S rRNA for the genus and species identification.

Results: A total of 50 (35.2%) actinomycetes isolates from 7 genera were isolated from 142 hospital environmental samples. The three most prevalent species were M. setense 10%, R. erythropolis and M. fortuitum 8% followed by N.cyriacigeorgica and M. gordonae 6%, M. chelonae, M. abscessus, M. lentiflavum, M. mucogenicum, N. asteroides, N. farcinica, R. equi and L. shinushuensis 4% and the single isolates of M. conceptionense, M. septicum, N. rhamnosophilia, N. bravicatena, M. flavescens, M. arupense, M. doricum, M. frederiksbergense, S. heliomycini, S. albus, S. albogriseolus, R. facians, D. maris, G. terae and A. globiformis.

Conclusions: In conclusion we showed that the hospital environment is a potential reservoir for a broad range of actinomycetes species, due to the remarkable survival capability of these microorganisms in adverse hospital environment, carrying a threat to the health of patients.

Keywords: 16S rRNA; Actinomycetes; Hospital environment; Nosocomial infection.

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Conflict of interest statement

The authors declare that there are no conflicts of interest.

Figures

Fig. 1
Fig. 1
Geographic distribution of sampling site from markazi province hospitals, blue spots are negative and Red spot are positive samples for isolation of actinomycetes. The Figure source is from Iran National mapping agency and designed by Adobe Photoshop 2020 v21.2.2.289
Fig. 2
Fig. 2
16S rRNA sequence based phylogenetic tree for environmental actionmycetes and nearest validated species of actinomycetes by using the neighbor-joining method. The figures at each node represent bootstrapping values
See this image and copyright information in PMC

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