Complete Genome Sequences of 12 Quinolone-Resistant Escherichia coli Strains Containing qnrS1 Based on Hybrid Assemblies
- PMID: 33509985
- PMCID: PMC7844070
- DOI: 10.1128/MRA.01190-20
Complete Genome Sequences of 12 Quinolone-Resistant Escherichia coli Strains Containing qnrS1 Based on Hybrid Assemblies
Abstract
In total, 12 quinolone-resistant Escherichia coli (QREC) strains containing qnrS1 were submitted to long-read sequencing using a FLO-MIN106 flow cell on a MinION device. The long reads were assembled with short reads (Illumina) and analyzed using the MOB-suite pipeline. Six of these QREC genome sequences were closed after hybrid assembly.
Copyright © 2021 Kaspersen et al.
Similar articles
-
Evaluation of strategies for the assembly of diverse bacterial genomes using MinION long-read sequencing.BMC Genomics. 2019 Jan 9;20(1):23. doi: 10.1186/s12864-018-5381-7. BMC Genomics. 2019. PMID: 30626323 Free PMC article.
-
Evaluation and Validation of Assembling Corrected PacBio Long Reads for Microbial Genome Completion via Hybrid Approaches.PLoS One. 2015 Dec 7;10(12):e0144305. doi: 10.1371/journal.pone.0144305. eCollection 2015. PLoS One. 2015. PMID: 26641475 Free PMC article.
-
High precision genome sequencing of engineered Gluconobacter oxydans 621H by combining long nanopore and short accurate Illumina reads.J Biotechnol. 2017 Sep 20;258:197-205. doi: 10.1016/j.jbiotec.2017.04.016. Epub 2017 Apr 19. J Biotechnol. 2017. PMID: 28433722
-
Genome assembly using Nanopore-guided long and error-free DNA reads.BMC Genomics. 2015 Apr 20;16(1):327. doi: 10.1186/s12864-015-1519-z. BMC Genomics. 2015. PMID: 25927464 Free PMC article.
-
Resolving plasmid structures in Enterobacteriaceae using the MinION nanopore sequencer: assessment of MinION and MinION/Illumina hybrid data assembly approaches.Microb Genom. 2017 Jun 9;3(8):e000118. doi: 10.1099/mgen.0.000118. eCollection 2017 Aug. Microb Genom. 2017. PMID: 29026658 Free PMC article.
Cited by
-
Longitudinal Sampling Reveals Persistence of and Genetic Diversity in Extended-Spectrum Cephalosporin-Resistant Escherichia coli From Norwegian Broiler Production.Front Microbiol. 2021 Dec 10;12:795127. doi: 10.3389/fmicb.2021.795127. eCollection 2021. Front Microbiol. 2021. PMID: 34956163 Free PMC article.
References
-
- Kaspersen H, Sekse C, Zeyl Fiskebeck E, Slettemeas JS, Simm R, Norstrom M, Urdahl AM, Lagesen K. 2020. Dissemination of quinolone-resistant Escherichia coli in the Norwegian broiler and pig production chain and possible persistence in the broiler production environment. Appl Environ Microbiol 86:e02769-19. doi:10.1128/AEM.02769-19. - DOI - PMC - PubMed
-
- Ueno Y, Arita M, Kumagai T, Asai K. 2003. Processing sequence annotation data using the Lua programming language. Genome Inform 14:154–163. - PubMed
LinkOut - more resources
Full Text Sources
Other Literature Sources