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Comment
. 2021 Jan 29;6(1):39.
doi: 10.1038/s41392-021-00470-1.

Engaging the spikes: heparan sulfate facilitates SARS-CoV-2 spike protein binding to ACE2 and potentiates viral infection

Rajkumar Singh Kalra  1   2 , Ramesh Kandimalla  3   4
Affiliations
Comment

Engaging the spikes: heparan sulfate facilitates SARS-CoV-2 spike protein binding to ACE2 and potentiates viral infection

Rajkumar Singh Kalra et al. Signal Transduct Target Ther. .
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Heparan sulfate enhances SARS-CoV-2 spike protein binding to ACE2 and potentiates viral infection. a SARS-CoV-2 virion and spike (S) glycoprotein. b A 1273-amino acid-long SARS-CoV-2 S glycoprotein. Receptor-binding domain (RBD) that interacts with the host cell surface ACE2 receptor is exhibited here in yellow. A network of hydrophilic (13H bonds and two salt bridges—shown in cyan) interactions contribute to this binding of ACE2 to RBD (Lan et al. 2020), whereas a putative HS-binding site is shown just adjacent to the ACE2-binding site in RBD. The key residues suggested to involve in the interaction are shown in green on the right of RBD. c. Schematic diagram showing HS-led open conformation of RBD and subsequent spike(S)-HS-ACE2 interaction. d Schematic model depicting SARS-COV-2 recognition and entry
See this image and copyright information in PMC

Comment on

  • SARS-CoV-2 Infection Depends on Cellular Heparan Sulfate and ACE2.
    Clausen TM, Sandoval DR, Spliid CB, Pihl J, Perrett HR, Painter CD, Narayanan A, Majowicz SA, Kwong EM, McVicar RN, Thacker BE, Glass CA, Yang Z, Torres JL, Golden GJ, Bartels PL, Porell RN, Garretson AF, Laubach L, Feldman J, Yin X, Pu Y, Hauser BM, Caradonna TM, Kellman BP, Martino C, Gordts PLSM, Chanda SK, Schmidt AG, Godula K, Leibel SL, Jose J, Corbett KD, Ward AB, Carlin AF, Esko JD. Clausen TM, et al. Cell. 2020 Nov 12;183(4):1043-1057.e15. doi: 10.1016/j.cell.2020.09.033. Epub 2020 Sep 14. Cell. 2020. PMID: 32970989 Free PMC article.

References

    1. Clausen TM, et al. SARS-CoV-2 infection depends on cellular heparan sulfate and ACE2. Cell. 2020;183:1–15. doi: 10.1016/j.cell.2020.09.033. - DOI - PMC - PubMed
    1. Lan J, et al. Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor. Nature. 2020;581:215–220. doi: 10.1038/s41586-020-2180-5. - DOI - PubMed
    1. Kim SY, et al. Characterization of heparin and severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) spike glycoprotein binding interactions. Antiviral Res. 2020;181:104873. doi: 10.1016/j.antiviral.2020.104873. - DOI - PMC - PubMed
    1. Lang J, et al. Inhibition of SARS pseudovirus cell entry by lactoferrin binding to heparan sulfate proteoglycans. PLoS ONE. 2011;6:e23710. doi: 10.1371/journal.pone.0023710. - DOI - PMC - PubMed
    1. Kwon PS, et al. Sulfated polysaccharides effectively inhibit SARS-CoV-2 in vitro. Cell Discov. 2020;6:50. doi: 10.1038/s41421-020-00192-8. - DOI - PMC - PubMed

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