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. 2021 Feb;100(2):675-684.
doi: 10.1016/j.psj.2020.10.043. Epub 2020 Nov 5.

Effects of ecofriendly synthesized calcium nanoparticles with biocompatible Sargassum latifolium algae extract supplementation on egg quality and scanning electron microscopy images of the eggshell of aged laying hens

Hayam A Abo El-Maaty  1 , Ayman Y El-Khateeb  2 , Hanan Al-Khalaifah  3 , El-Sayed A El Hamed  4 , Sahar Hamed  5 , Eman A El-Said  6 , Khalid M Mahrose  7 , Khaled Metwally  8 , Amira M Mansour  1
Affiliations

Effects of ecofriendly synthesized calcium nanoparticles with biocompatible Sargassum latifolium algae extract supplementation on egg quality and scanning electron microscopy images of the eggshell of aged laying hens

Hayam A Abo El-Maaty et al. Poult Sci. 2021 Feb.

Abstract

In the present study, 200 Brown commercial egg-type layers (60 wk old) were used to study the effects of different levels of ecofriendly synthesis of calcium (Ca) nanoparticles (0.0, 0.50, 1.0, and 1.5 g/kg diet) with biocompatible Sargassum latifolium algae extract (SL-CaNps) on exterior egg quality traits, electronic microscopic view of eggshells, Ca and phosphorus (P) retention, serum Ca and P concentrations, and the histology of the uterus. Hens fed with dietary SL-CaNps powder had higher egg weight and shell weight % values than those of the control group. All SL-CaNps treatment groups had the greatest values of shell weight per unit surface area and shell thickness. Dietary supplementation of SL-CaNps at graded levels up to 1.5 g/kg diet had higher serum Ca and inorganic P levels than that of the control. Laying hens fed with SL-CaNps-added diets had beneficial effects on shell ultrastructure in terms of well-developed palisade and mammillary layers. The numbers of apical cells along the branched tubular gland were greater in SL-CaNps-treated groups than those of control. Conclusively, supplementing SL-CaNps powder up to 1.5 g/kg to the diet of laying hens improved eggshell thickness, shell weight% and shell weight per unit surface and has no adverse effect on their eggshell quality or electronic microscopic view of their eggshell.

Keywords: SL-CaNps powder; eggshell quality; electronic microscopic view of eggshell; laying hens; ultrastructure.

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Figures

Figure 1
Figure 1
UV-Vis spectroscopic measurements of Sargassum latifolium algae and its calcium nanoparticles.
Figure 2
Figure 2
Transmission electron microscope micrographs and size distributions for calcium nanoparticles synthesized by algae extract at 100 and 200 nm magnification value.
Figure 3
Figure 3
Zeta potential values for calcium nanoparticles synthesized by algae extract.
Figure 4
Figure 4
(A) Scanning electron microscopic image of tissues from the control group. (B) Scanning electron microscopic image of tissues from hens fed with 0.5 g of Ca nanoparticles with biocompatible Sargassum latifolium algae extract (SL-CaNps). (C) Scanning electron microscopic image of tissues from hens fed with 1.0 g Ca nanoparticles with biocompatible S. latifolium algae extract (SL-CaNps). (D) Scanning electron microscopic image of tissues from hens fed with 1.5 g of Ca nanoparticles with biocompatible S. latifolium algae extract (SL-CaNps).
Figure 5
Figure 5
(A) Histologic of uterus samples from the control group. (B) Histologic of Uterus samples from hens fed with 0.5 g of Ca nanoparticles with biocompatible Sargassum latifolium algae extract (SL-CaNps). (C) Histologic of uterus samples from hens fed with 1.0 g of Ca nanoparticles with biocompatible S. latifolium algae extract (SL-CaNps). (D) Histologic of uterus samples from hens fed with 1.5 g of Ca nanoparticles with biocompatible S. latifolium algae extract (SL-CaNps).
See this image and copyright information in PMC

References

    1. Abd El-Ghany W. Nanotechnology and its considerations in poultry field: an overview. J. Hellenic Vet. Med. Soc. 2019;70:1611–1616.
    1. Abd El-Hack M.E., Alagawany M., Mahrose Kh.M., Arif M., Saeed M., Arain M.A., Soomro R.N., Siyal F.A., Fazlani S.A., Fowler J. Laying performance, physical, and internal egg quality criteria of hens fed distillers dried grains with solubles and exogenous enzyme mixture. Anim. Nutr. 2019;5:49–55. - PMC - PubMed
    1. Abd El-Hack M.E., Chaudhry M.T., Mahrose Kh.M., Noreldin A., Emam M., Alagawany M. The efficacy of using exogenous enzymes cocktail on production, egg quality, egg nutrients and blood metabolites of laying hens fed distiller's dried grains with solubles. J. Anim. Physiol. Anim. Nutr. 2018;102:e726–e735. - PubMed
    1. Abd El-Hack M.E., Mahrose K.M., Attia F.A.M., Swelum A.A., Taha A.E., Shewita R.S., Hussein E.S.O.S., Alowaimer A.N. Laying performance, physical, and internal egg quality criteria of hens fed distillers dried grains with solubles and exogenous enzyme mixture. Animals (Basel) 2019;9:150. - PMC - PubMed
    1. Alagawany M., Mahrose Kh.M. Influence of different levels of certain essential amino acids on the performance, egg quality criteria and economics of Lohmann Brown laying hens. Asian J. Poult. Sci. 2014;8:82–96.