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Multicenter Study
. 2020 Nov 3:37:213.
doi: 10.11604/pamj.2020.37.213.24988. eCollection 2020.

Prevalence of extended-spectrum β-lactamase (ESBL) and molecular detection of bla TEM, bla SHV and bla CTX-M genotypes among Enterobacteriaceae isolates from patients in Khartoum, Sudan

Maha Hassan Dirar  1 , Naser Eldin Bilal  1 , Mutasim Elhadi Ibrahim  2 , Mohamed Elamin Hamid  3
Affiliations
Multicenter Study

Prevalence of extended-spectrum β-lactamase (ESBL) and molecular detection of bla TEM, bla SHV and bla CTX-M genotypes among Enterobacteriaceae isolates from patients in Khartoum, Sudan

Maha Hassan Dirar et al. Pan Afr Med J. .

Abstract

Introduction: the emergence of antibiotic resistance pathogens is an important health risk. Usually Gram negative bacteria acquire resistance to beta-lactam antibiotics by beta-lactamase production. The objectives of this study was to assess the prevalence of ESBL and to detect the frequency of blaTEM, blaSHV and blaCTX-M genotypes among ESBL producing Enterobacteriaceae isolates from patients in Khartoum, Sudan.

Methods: a total of 171 isolates of Enterobacteriaceae were recovered from hospitals in Khartoum, Sudan (2014 -2015) were used to detect ESBL production using disc diffusion method. blaTEM, blaSHV and blaCTX-M genes were investigated by PCR based methods using gene-specific primers.

Results: the high resistance among Enterobacteriaceae was noticed in ciprofloxacin (72%) and ofloxacin (73%). ESBL production was mainly in Escherichia Coli (38%) and Klebsiella pneumonia (34%). Prevalent genotypes were blaTEM (86%), blaCTX-M (78%) and blaSHV (28%). These were found mainly in Escherichia Coli (38%, 37%, 2%) and K. pneumonia (34%, 31%, 26.1%). The majority of ESBL producing isolates possess more than one ESBL genes.

Conclusion: the ESBL production in Enterobacteriaceae was high, with blaTEM and blaCTX-M genotypes more prevalent. Public health and laboratory standard of excellence is needed to reducing the spread of resistant pathogens.

Keywords: Antimicrobial resistance; Escherichia coli; Gram negative bacteria; Klebsiella pneumoniae; genotyping.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
detection of blaTEM, blaSHV and blaCTX-M genotypes in clinical isolates of the family Enterobacteriaceae from patients in Khartoum, Sudan (2014-2015)
Figure 2
Figure 2
PCR assays for the detection of genotypes from phenotypically ESBL positive isolates; blaTEM (931 bp) in the upper gel: lanes 1, 3-10 were blaTEM positive isolates and lane 2, negative control isolate; blaSHV (868 bp) in the middle gel: lanes 1, 2, 3 and 6 were blaSHV positive isolates; lanes 5, 7-10 were blaSHV negative isolates and lane 4, a negative control isolate; blaCTX-M (909 bp) in the lower gel: lanes 1, 4, 7-10 were blaCTX-M positive isolates; lanes 3, 5, 6 were blaCTX-M negative isolates and lane 2, negative control isolate; Lane M represents the molecular weight marker (1 kb DNA Ladder, Promega)
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