Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jun;3(1):1-3.
doi: 10.1016/j.jobb.2020.12.001. Epub 2021 Jan 23.

Heat inactivation of the severe acute respiratory syndrome coronavirus 2

Christophe Batéjat  1 , Quentin Grassin  1 , Jean-Claude Manuguerra  1 , India Leclercq  1   2
Affiliations

Heat inactivation of the severe acute respiratory syndrome coronavirus 2

Christophe Batéjat et al. J Biosaf Biosecur. 2021 Jun.

Abstract

Cell culture medium, nasopharyngeal and sera samples spiked with SARS-CoV-2 were subjected to heat inactivation for various periods of time, ranging from 30 s to 60 min. Our results showed that SARS-CoV-2 could be inactivated in less than 30 min, 15 min, and 3 min at 56 °C, 65 °C, and 95 °C, respectively. These data could help laboratory workers to improve their protocols by handling the virus in biosafety conditions.

Keywords: Covid-19; Heat; Inactivation; SARS-CoV-2.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Viral titers in log10 TCID50/mL obtained after heat inactivation of infected cell culture medium (A), nasopharyngeal samples spiked with SARS-CoV-2 (B), and sera samples from negative donors spiked with SARS-CoV-2 (C). Each condition was performed in triplicate. Each dot represents the mean viral titer and vertical lines represent the standard deviation.
See this image and copyright information in PMC

References

    1. WHO-COVID-19-lab_testing-2020.1-eng.pdf. Accessed April 28, 2020. https://apps.who.int/iris/bitstream/handle/10665/331509/WHO-COVID-19-lab...
    1. Cutts T., Grolla A., Jones S., Cook B.W.M., Qiu X., Theriault S.S. Inactivation of Zaire ebolavirus variant Makona in human serum samples analyzed by enzyme-linked immunosorbent assay. J Infect Dis. 2016;214(suppl 3):S218–S221. doi: 10.1093/infdis/jiw289. - DOI - PubMed
    1. Park S.L., Huang Y.-J.S., Hsu W.-W., Hettenbach S.M., Higgs S., Vanlandingham D.L. Virus-specific thermostability and heat inactivation profiles of alphaviruses. J Virol Methods. 2016;234:152–155. doi: 10.1016/j.jviromet.2016.04.004. - DOI - PubMed
    1. Edwards S. Survival and inactivation of classical swine fever virus. Vet Microbiol. 2000;73(2):175–181. doi: 10.1016/S0378-1135(00)00143-7. - DOI - PubMed
    1. Leclercq I., Batéjat C., Burguière A.M., Manuguerra J.-C. Heat inactivation of the Middle East respiratory syndrome coronavirus. Influenza Other Respir Viruses. 2014;8(5):585–586. doi: 10.1111/irv.12261. - DOI - PMC - PubMed