Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Oct;64(10):1661-1676.
doi: 10.1007/s11427-020-1836-7. Epub 2021 Jan 27.

Longitudinal single-cell RNA-seq of hESCs-derived retinal organoids

Shaojun Wang  1   2 , Sergio Poli  3   4 , Xiaoliang Liang  3 , Guang-Hua Peng  5   6
Affiliations

Longitudinal single-cell RNA-seq of hESCs-derived retinal organoids

Shaojun Wang et al. Sci China Life Sci. 2021 Oct.

Abstract

Human retina development involves multiple well-studied signaling pathways that promote the genesis of a wide arrange of different cell types in a complex architectural structure. Human embryonic stem cells (hESCs)-derived retinal organoids could recapitulate the human retinal development. We performed single-cell RNA-seq of retinal organoids from 5 time points (D36, D66, D96, D126, D186) and identified 9 distinct populations of cells. In addition, we analyzed the molecular characteristics of each main population and followed them from genesis to maturity by pseudotime analysis and characterized the cell-cell interactions between different cell types. Interestingly, we identified insulin receptor (INSR) as a specifically expressed receptor involved in the genesis of photoreceptors, and pleiothropin (PTN)-protein tyrosine phosphatase receptor type Z1 (PTPRZ1) as a mediator of a previously unknown interaction between Müller and retinal progenitor cells. Taken together, these findings provide a rich transcriptome-based lineage map for studying human retinal development and modeling developmental disorders in retinal organoids.

Keywords: human embryonic stem cell; photoreceptor cell; retinal organoid; retinal pigment epithelium; single cell RNA sequencing.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Berson, D. (2003). Strange vision: ganglion cells as circadian photoreceptors. Trends Neurosci 26, 314–320. - PubMed - DOI - PMC
    1. Butler, A., Hoffman, P., Smibert, P., Papalexi, E., and Satija, R. (2018). Integrating single-cell transcriptomic data across different conditions, technologies, and species. Nat Biotechnol 36, 411–420. - PubMed - PMC - DOI
    1. Butler, J.M., Sharif, U., Ali, M., McKibbin, M., Thompson, J.P., Gale, R., Yang, Y.C., Inglehearn, C., and Paraoan, L. (2015). A missense variant in CST3 exerts a recessive effect on susceptibility to age-related macular degeneration resembling its association with Alzheimer’s disease. Hum Genet 134, 705–715. - PubMed - PMC - DOI
    1. Camp, J.G., Wollny, D., and Treutlein, B. (2018). Single-cell genomics to guide human stem cell and tissue engineering. Nat Methods 15, 661–667. - PubMed - DOI - PMC
    1. Carlin, D.E., Demchak, B., Pratt, D., Sage, E., and Ideker, T. (2017). Network propagation in the cytoscape cyberinfrastructure. PLoS Comput Biol 13, e1005598. - PubMed - PMC - DOI

MeSH terms

Substances

LinkOut - more resources