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. 2021 Jul;40(7):1553-1557.
doi: 10.1007/s10096-021-04168-8. Epub 2021 Feb 1.

The yield of CSF molecular testing in febrile neonates

Basheer Nassrallah #  1 , Ellen Bamberger #  1   2 , Sarah Cohen  3 , Isaac Srugo  1   2   4 , Orit Golan-Shany  4 , Yulia Shlonsky  4 , Raeda Mubariki  4 , Jacob Genizi  5   6   7
Affiliations

The yield of CSF molecular testing in febrile neonates

Basheer Nassrallah et al. Eur J Clin Microbiol Infect Dis. 2021 Jul.

Abstract

We retrospectively examined the yield of a cerebrospinal fluid (CSF) multiplex real-time PCR assay of febrile young infants undergoing a full sepsis work-up. Eighty infants were included in the study: Forty-nine (61%) neonates and 31 (39%) 29-90 day-old patients were included in the study. A viral pathogen was detected in 59% (47/80) of the samples, human enterovirus in 53% (42/80) and Human parechovirus in 6% (5/80). The CSF of nearly half of the subjects with CNS infection was without pleocytosis; all CSF cultures were negative. Multiplex PCR CSF testing enhances the diagnosis of pathogen-specific viral CNS infection among febrile young infants.

Keywords: Infants; Infection; Meningitis; Multiplex PCR.

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References

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