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. 2021 Jan 28;26(3):690.
doi: 10.3390/molecules26030690.

Are the Closely Related Cobetia Strains of Different Species?

Yulia Noskova  1 , Aleksandra Seitkalieva  1 , Olga Nedashkovskaya  1 , Liudmila Shevchenko  1 , Liudmila Tekutyeva  2 , Oksana Son  2 , Larissa Balabanova  1   2
Affiliations

Are the Closely Related Cobetia Strains of Different Species?

Yulia Noskova et al. Molecules. .

Abstract

Marine bacteria of the genus Cobetia, which are promising sources of unique enzymes and secondary metabolites, were found to be complicatedly identified both by phenotypic indicators due to their ecophysiology diversity and 16S rRNA sequences because of their high homology. Therefore, searching for the additional methods for the species identification of Cobetia isolates is significant. The species-specific coding sequences for the enzymes of each functional category and different structural families were applied as additional molecular markers. The 13 closely related Cobetia isolates, collected in the Pacific Ocean from various habitats, were differentiated by the species-specific PCR patterns. An alkaline phosphatase PhoA seems to be a highly specific marker for C. amphilecti. However, the issue of C. amphilecti and C. litoralis, as well as C. marina and C. pacifica, belonging to the same or different species remains open.

Keywords: Cobetia amphilecti; Cobetia crustatorum; Cobetia litoralis; Cobetia marina; Cobetia pacifica; alkaline phosphatase PhoA; identification markers.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Gel-electrophoresis of PCR products of the new molecular markers: (A) C. amphilecti KMM 1561T, C. amphilecti KMM 296 and KMM 7516; (B) C. marina LMG 2217T and KMM 6284; (C) C. litoralis KMM 3880T, KMM 7000, KMM 7005 and KMM 7514; (D) C. pacifica KMM 3879T, KMM 6731, 7505, 7508, 7515, 6816, and 6818. Lane numbers: 1—DNA/RNA non-specific endonuclease precursor; 2—DNA/RNA non-specific endonuclease; 3—EEP-like (DNaseI-like) nuclease; 4—alkaline phosphatase/phosphodiesterase PhoD; 5—phospholipase A; 6—ATP-dependent protease Clp; 7—periplasmic serine peptidase Do/DeqQ; 8—CmAP-like alkaline phosphatase PhoA; M—1 kb DNA ladder marker (Evrogen).
Figure 2
Figure 2
Gel electrophoresis of PCR products of the new molecular markers in the strains C. crustatorum JCM 15644T and C. crustatorum KMM 6817. Lane numbers: 1—DNA/RNA non-specific endonuclease precursor; 2—DNA/RNA non-specific endonuclease; 3—EEP-like (DNaseI-like) nuclease; 4—alkaline phosphatase/phosphodiesterase PhoD; 5—phospholipase A; 6—ATP-dependent protease Clp; 7—periplasmic serine peptidase Do/DeqQ; 8—CmAP-like alkaline phosphatase PhoA; M—1 kb DNA ladder marker (Evrogen).
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