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. 1988 Feb;8(2):764-9.
doi: 10.1128/mcb.8.2.764-769.1988.

Amplification and expression of heterologous ornithine decarboxylase in Chinese hamster cells

T R Chiang  1 , L McConlogue
Affiliations

Amplification and expression of heterologous ornithine decarboxylase in Chinese hamster cells

T R Chiang et al. Mol Cell Biol. 1988 Feb.

Abstract

We have developed an amplifiable mammalian expression vector based on the enzyme ornithine decarboxylase (ODC). We show greater than 700-fold amplification of this vector in ODC-deficient Chinese hamster ovary cells. A passive coamplified marker, dihydrofolate reductase (dhfr), was amplified and overexpressed 1,000-fold. This ODC vector was a dominant marker in a variety of cell types and displayed at least 300-fold amplification in wild-type Chinese hamster ovary cells.

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References

    1. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 - PubMed
    1. Mol Cell Biol. 1984 Jan;4(1):166-72 - PubMed
    1. Cell. 1981 Jan;23(1):29-39 - PubMed
    1. Annu Rev Biochem. 1984;53:749-90 - PubMed
    1. Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 - PubMed

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