Regulatory effects of Lactobacillus plantarum-GMNL6 on human skin health by improving skin microbiome

Abstract

Bacteria response to their environment by producing some compounds which are used in cosmetic and pharmaceutical applications. Some probiotics can regulate immune response and modulate the symptoms of several diseases. Bacteria affect skin response to skin care products. Bacteria are thought to play an important role in acne incidence, skin moisture, and nutrient metabolism, but only a few studies have focused on the extracts of Lactobacillus plantarum in skin care. In this study, we identified that L. plantarum-GMNL6 enhanced collagen synthesis and the gene expression of serine palmitoyltransferase small subunit A. Meanwhile, L. plantarum-GMNL6 reduced the melanin synthesis, the biofilm of Staphylococcus aureus, and the proliferation of Cutibacterium acnes. Information from clinical observation during the ointment for external face use in people displayed that the syndromes of skin moisture, skin color, spots, wrinkles, UV spots, and porphyrins were improved. The diversification of human skin microbiomes was affected by smearing the face of volunteers with L. plantarum-GMNL6. Understanding the potential mechanisms of the action of L. plantarum-GMNL6 in dermatologic conditions promotes the development of care products.

Keywords: Lactobacillus plantarum-GMNL6; care products; probiotics; skin care.

Figure 1

Effects of various probiotic on skin health. HS68 cells were treated with various probiotics (5× 10⁸ cells/ml) after 24 h. (A) The supernatant was collected and then analyzed collagens. (B) The cell extracts were collected, RNA was extracted and converted into cDNA, and the serine palmitoyltransferase small subunit A (SPTSSA) and the β-actin were quantified by real-time-PCR analysis.

Figure 2

The collagen synthesis, the gene expression of SPTSSA, melanogenesis, and biofilm formation were measured after lipoteichoic acid (LTA) and peptidoglycan (PGN) of L. plantarum-GMNL6 treatment. Effect of LTA and PGN on (A) cellular collage synthesis and (B) the gene expression of SPTSSA. (C) Effect of lipoteichoic acid (LTA) and peptidoglycan (PGN) on cellular melanin content. (D)The inhibition of biofilm formation of S. aureus was measured after LTA and PGN treatment. (n = 6, data are mean± SD. * P < 0.05, ^# P < 0.01).

Figure 3

Recruitment of participants and the study design. The cream containing probiotics or not was performed a total of 2 months. All participates were requested to collect data by using Corneometer^® CM 825, Skicon 200 EX® Derma-Spectrometer, and VISIA^®.

Figure 4

Comparison with the skin microbiomes before and after using L. plantarum-GMNL6 treatment. The relative abundance of five major genera in skin microbiomes is compared before and after using L. plantarum-GMNL6 treatment. Statistically significant P-values are shown in red.