Hyperglycemia-Induced Cardiac Damage Is Alleviated by Heat-Inactivated Lactobacillus reuteri GMNL-263 via Activation of the IGF1R Survival Pathway

Ker-Ping Koay^#¹, Bruce Chi-Kang Tsai², Chia-Hua Kuo³, Wei-Wen Kuo^#^{4

5}, Hsiang-Ning Luk¹, Cecilia Hsuan Day⁶, Ray-Jade Chen⁷, Michael Yu-Chih Chen⁸, V Vijaya Padma⁹, Chih-Yang Huang^{10

11

12

13

14}

Affiliations

¹ Department of Anesthesia, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
² Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
³ Laboratory of Exercise Biochemistry, University of Taipei, Taipei, Taiwan.
⁴ Department of Biological Science and Technology, College of Life Sciences, China Medical University, Taichung, Taiwan.
⁵ Ph.D. Program for Biotechnology Industry, China Medical University, Taichung, Taiwan.
⁶ Department of Nursing, MeiHo University, Pingtung, Taiwan.
⁷ Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
⁸ Department of Cardiology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
⁹ Department of Biotechnology, Bharathiar University, Coimbatore, India.
¹⁰ Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan. cyhuang@mail.cmu.edu.tw.
¹¹ Department of Biological Science and Technology, Asia University, Taichung, Taiwan. cyhuang@mail.cmu.edu.tw.
¹² Center of General Education, Tzu Chi University of Science and Technology, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan. cyhuang@mail.cmu.edu.tw.
¹³ Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan. cyhuang@mail.cmu.edu.tw.
¹⁴ Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan. cyhuang@mail.cmu.edu.tw.

^# Contributed equally.

PMID: 33527184
DOI: 10.1007/s12602-021-09745-z

Hyperglycemia-Induced Cardiac Damage Is Alleviated by Heat-Inactivated Lactobacillus reuteri GMNL-263 via Activation of the IGF1R Survival Pathway

Ker-Ping Koay et al. Probiotics Antimicrob Proteins. 2021 Aug.

. 2021 Aug;13(4):1044-1053.

doi: 10.1007/s12602-021-09745-z. Epub 2021 Feb 1.

Authors

Affiliations

¹ Department of Anesthesia, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
² Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
³ Laboratory of Exercise Biochemistry, University of Taipei, Taipei, Taiwan.
⁴ Department of Biological Science and Technology, College of Life Sciences, China Medical University, Taichung, Taiwan.
⁵ Ph.D. Program for Biotechnology Industry, China Medical University, Taichung, Taiwan.
⁶ Department of Nursing, MeiHo University, Pingtung, Taiwan.
⁷ Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
⁸ Department of Cardiology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
⁹ Department of Biotechnology, Bharathiar University, Coimbatore, India.
¹⁰ Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan. cyhuang@mail.cmu.edu.tw.
¹¹ Department of Biological Science and Technology, Asia University, Taichung, Taiwan. cyhuang@mail.cmu.edu.tw.
¹² Center of General Education, Tzu Chi University of Science and Technology, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan. cyhuang@mail.cmu.edu.tw.
¹³ Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan. cyhuang@mail.cmu.edu.tw.
¹⁴ Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan. cyhuang@mail.cmu.edu.tw.

^# Contributed equally.

PMID: 33527184
DOI: 10.1007/s12602-021-09745-z

Abstract

Diabetes-induced cardiomyocyte apoptosis is one of the major causes of mortality in patients with diabetes. Numerous studies have indicated the beneficial effects of Lactobacillus reuteri GMNL-263. However, the protective effect of Lactobacillus reuteri GMNL-263 in cardiac damage associated with diabetes remains poorly understood. In this study, we aimed to investigate the protective effect of Lactobacillus reuteri GMNL-263 on cardiomyocytes in diabetic rats. Five-week-old male Wistar rats were categorized into normal control group, diabetes group (55 mg/kgw STZ-induced diabetes via intraperitoneal injection), and diabetic animals treated with Lactobacillus reuteri GMNL-263 (10⁹ CFU/rat/day, oral administration for 4 weeks). The results were presented that oral administration of a high dose of Lactobacillus reuteri GMNL-263 in diabetic rats activated IGF1R cell survival pathways to decrease the Fas-dependent and mitochondrial-dependent apoptotic pathways induced by hyperglycemia. We found that GMNL-263 significantly attenuated cell apoptosis via the IGF1R survival pathway in diabetic rats. The findings of this study suggest that GMNL-263 treatment maybe an effective therapeutic approach for the prevention of cardiac apoptosis in patients with diabetes.

Keywords: Diabetes; GMNL-263; Heart; Hyperglycemia; IGF1R.

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References

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1. Bailes BK (2002) Diabetes mellitus and its chronic complications. AORN J 76:265–282. https://doi.org/10.1016/S0001-2092(06)61065-X - DOI
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Hyperglycemia-Induced Cardiac Damage Is Alleviated by Heat-Inactivated Lactobacillus reuteri GMNL-263 via Activation of the IGF1R Survival Pathway

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Hyperglycemia-Induced Cardiac Damage Is Alleviated by Heat-Inactivated Lactobacillus reuteri GMNL-263 via Activation of the IGF1R Survival Pathway

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Abstract

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Medical

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