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. 2021 Mar;35(2):993-996.
doi: 10.1111/jvim.16040. Epub 2021 Feb 1.

Diagnostic accuracy of a point-of-care test using voided urine samples for detection of bacteriuria in dogs with signs of lower urinary tract disease

David C Grant  1 , Michael T Nappier  1 , Virginia Kiefer Corrigan  1
Affiliations

Diagnostic accuracy of a point-of-care test using voided urine samples for detection of bacteriuria in dogs with signs of lower urinary tract disease

David C Grant et al. J Vet Intern Med. 2021 Mar.

Abstract

Background: Bacterial urine culture is recommended in dogs suspected of having urinary tract infection (UTI), but there is expense and delay in obtaining such results.

Hypothesis/objective: To determine the diagnostic performance of a rapid immunoassay (RIA) dipstick for detection of bacteriuria using voided urine from dogs with clinical signs of lower UTI.

Animals: Twenty-four client-owned dogs.

Methods: Voided urine was collected and the RIA performed within 30 minutes. Urine collected by cystocentesis was submitted for aerobic urine culture. McNemar's test and kappa coefficient were calculated to determine agreement between the 2 tests.

Results: Nine of 21 dogs (43%) had UTI verified by aerobic urine culture. There was 1 false-negative and no false-positive RIA results. Sensitivity, specificity, positive predictive value, and negative predictive value of the RIA were 89%, 100%, 100%, and 92%, respectively.

Conclusions and clinical importance: This RIA is promising for correctly identifying whether or not voided urine samples from dogs with lower urinary tract clinical signs have true bacteriuria in a rapid, inexpensive manner. Additional patients should be enrolled in a similar study to determine if diagnostic performance is robust in a large population.

Keywords: bladder; cystitis; infection; urinary tract; urine culture.

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Conflict of interest statement

Authors declare no conflict of interest.

References

    1. Weese JS, Blondeau J, Boothe D, et al. International Society for Companion Animal Infectious Diseases (ISCAID) guidelines for the diagnosis and management of bacterial urinary tract infections in dogs and cats. Vet J. 2019;247:8‐25. - PubMed
    1. Ling GV, Ruby AL. Aerobic bacterial flora of the prepuce, urethra, and vagina of normal adult dogs. Am J Vet Res. 1978;39:695‐698. - PubMed
    1. Fowler H, Davis MA, Perkins A, et al. A survey of veterinary antimicrobial prescribing practices, Washington State 2015. Vet Rec. 2016;179:651. - PubMed
    1. Swenson CL, Boisvert AM, Kruger JM, Gibbons‐Burgener SN. Evaluation of modified Wright‐staining of urine sediment as a method for accurate detection of bacteriuria in dogs. J Am Vet Med Assoc. 2004;224:1282‐1289. - PubMed
    1. Olin SJ, Bartges JW, Jones RD, Bemis DA. Diagnostic accuracy of a point‐of‐care urine bacteriologic culture test in dogs. J Am Vet Med Assoc. 2013;243:1719‐1725. - PubMed