Antibiofilm effect of C-10 massoia lactone toward polymicrobial oral biofilms

Abstract

This study is aimed to test the efficacy of C-10 Massoia lactone in oral polymicrobial degradation. Polymicrobial of Streptococcus sanguinis, Streptococcus mutans, Lactobacillus acidophilus, and Actinomyces viscosus were studied. C-10 Massoia lactone against biofilm degradation was investigated using modified crystal violet for biofilm staining. The effectiveness of C-10 Massoia lactone against biofilms was calculated by the minimum biofilm inhibitory concentration (MBIC₅₀) and the minimum value of biofilm eradication concentration (MBEC₅₀). Scanning electron microscope was used to study biofilm cell viability and morphological changes. The results showed a degradation effect of C-10 Massoia lactone against mature oral polymicrobial at 0.25% v/v. C-10 Massoia lactone can degrade polymicrobial biofilms of S. mutans, S. sanguinis, L. acidophilus and A. viscosus. This compound can destroy the extracellular polymeric substances (EPS) of polymicrobial biofilms. The potential application of C-10 Massoia lactone for anti-polymicrobial medication should be applied in such a way that any negative effects are minimized. Further research is needed to confirm the findings of this study.

Keywords: Actinomyces viscosus; C10 Massoia lactone; Lactobacillus acidophilus; Streptococcus spp; polymicrobial biofilms; scanning electron microscope.

Figure 1

Effect of C-10 Massoia lactone on biofilm degradation of polymicrobial for intermediate phase: (a), mature phase; (b) biofilm, statistical significance of difference was determined by one way ANOVA with post hoc Bonferroni. ns: not significant; *P < 0.05

Figure 2

Biofilm eradication activity of C10 Massoia lactone against S. mutans ATCC 10566 mature biofilm monitored by scanning electron microscope; (a) cells treated with C10 Massoia lactone at minimum value of biofilm eradication concentration (0.25%); 1: magnification ×1000; 2: magnification ×5000; (b) control (untreated) cells; 1: magnification ×1000; 2: magnification ×5000

Figure 3

Biofilm eradication activity of C10 massoia lactone against L. acidophilus ATCC 4356 mature biofilm monitored by scanning electron microscope; (a) cells treated with C10 Massoia lactone at minimum value of biofilm eradication concentration (1%), 1: magnification ×1000; 2: magnification ×5000; (b) control (untreated) cells; 1: magnification ×1000; 2: magnification ×5000

Figure 4

Biofilm eradication activity of C10 massoia lactone against A. viscosus ATCC 15987 mature biofilm monitored by scanning electron microscope; (a) cells treated with C10 Massoia lactone at MBEC (1%), 1: magnification ×1000; 2: magnification ×5000; (b) control (untreated) cells; 1: magnification ×1000; 2: magnification ×5000

Figure 5

Biofilm eradication activity of C10 Massoia lactone against polymicrobial mature biofilm monitored by scanning electron microscope; (a) cells treated with C10 Massoia lactone at MBEC (1%), 1: magnification ×1000; 2: magnification ×5000; (b) control (untreated) cells; 1: magnification ×1000; 2: magnification ×5000