Neutralization of Zika virus by E protein domain III-Specific human monoclonal antibody

Sang Il Kim¹, Sujeong Kim², Jung Min Shim³, Hyo Jung Lee⁴, So Young Chang⁵, Seoryeong Park⁶, Ji-Young Min⁷, Wan Beom Park⁸, Myoung-Don Oh⁹, Seungtaek Kim¹⁰, Junho Chung¹¹

Affiliations

¹ Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea. Electronic address: sangk1128@snu.ac.kr.
² Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea; Department of Biomedical Science, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea. Electronic address: sujeong5425@snu.ac.kr.
³ Zoonotic Virus Laboratory, Institut Pasteur Korea, Gyeonggi-do, 13488, Republic of Korea.
⁴ Zoonotic Virus Laboratory, Institut Pasteur Korea, Gyeonggi-do, 13488, Republic of Korea. Electronic address: heading1230@naver.com.
⁵ Respiratory Virus Laboratory, Institut Pasteur Korea, Gyeonggi-do, 13488, Republic of Korea. Electronic address: sychang0709@gmail.com.
⁶ Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea. Electronic address: ryeong0512@gmail.com.
⁷ Respiratory Virus Laboratory, Institut Pasteur Korea, Gyeonggi-do, 13488, Republic of Korea. Electronic address: ji-young.x.min@gsk.com.
⁸ Department of Internal Medicine, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea. Electronic address: wbpark1@snu.ac.kr.
⁹ Department of Internal Medicine, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea. Electronic address: mdohmd@snu.ac.kr.
¹⁰ Zoonotic Virus Laboratory, Institut Pasteur Korea, Gyeonggi-do, 13488, Republic of Korea. Electronic address: seungtaek.kim@ip-korea.org.
¹¹ Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea; Department of Biomedical Science, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea. Electronic address: jjhchung@snu.ac.kr.

PMID: 33535104
DOI: 10.1016/j.bbrc.2021.01.075

Free article

Neutralization of Zika virus by E protein domain III-Specific human monoclonal antibody

Sang Il Kim et al. Biochem Biophys Res Commun. 2021.

Free article

. 2021 Mar 19:545:33-39.

doi: 10.1016/j.bbrc.2021.01.075. Epub 2021 Jan 31.

Authors

Sang Il Kim¹, Sujeong Kim², Jung Min Shim³, Hyo Jung Lee⁴, So Young Chang⁵, Seoryeong Park⁶, Ji-Young Min⁷, Wan Beom Park⁸, Myoung-Don Oh⁹, Seungtaek Kim¹⁰, Junho Chung¹¹

Affiliations

¹ Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea. Electronic address: sangk1128@snu.ac.kr.
² Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea; Department of Biomedical Science, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea. Electronic address: sujeong5425@snu.ac.kr.
³ Zoonotic Virus Laboratory, Institut Pasteur Korea, Gyeonggi-do, 13488, Republic of Korea.
⁴ Zoonotic Virus Laboratory, Institut Pasteur Korea, Gyeonggi-do, 13488, Republic of Korea. Electronic address: heading1230@naver.com.
⁵ Respiratory Virus Laboratory, Institut Pasteur Korea, Gyeonggi-do, 13488, Republic of Korea. Electronic address: sychang0709@gmail.com.
⁶ Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea. Electronic address: ryeong0512@gmail.com.
⁷ Respiratory Virus Laboratory, Institut Pasteur Korea, Gyeonggi-do, 13488, Republic of Korea. Electronic address: ji-young.x.min@gsk.com.
⁸ Department of Internal Medicine, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea. Electronic address: wbpark1@snu.ac.kr.
⁹ Department of Internal Medicine, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea. Electronic address: mdohmd@snu.ac.kr.
¹⁰ Zoonotic Virus Laboratory, Institut Pasteur Korea, Gyeonggi-do, 13488, Republic of Korea. Electronic address: seungtaek.kim@ip-korea.org.
¹¹ Department of Biochemistry and Molecular Biology, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea; Cancer Research Institute, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea; Department of Biomedical Science, Seoul National University College of Medicine, Seoul, 03080, Republic of Korea. Electronic address: jjhchung@snu.ac.kr.

PMID: 33535104
DOI: 10.1016/j.bbrc.2021.01.075

Abstract

Zika virus (ZIKV) infection in both infants and adults is associated with neurological complications including, but not limited to, microcephaly and Guillain-Barre syndrome. Antibody therapy can be effective against virus infection. We isolated ZIKV envelope domain III-specific neutralizing antibodies (nAbs) from two convalescent patients with ZIKV infection. One antibody, 2F-8, exhibited potent in vitro neutralizing activity against Asian and American strains of ZIKV. To prevent FcγR-mediated antibody-dependent enhancement, we prepared IgG₁ with LALA variation. A single dose of 2F-8 in the context of IgG₁ or IgG₁-LALA prior to or post lethal ZIKV challenge conferred complete protection in mice.

Keywords: Antibody-dependent enhancement; Guillain-Barre syndrome; Microcephaly; Neutralizing antibody; Zika virus.

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Conflict of interest statement

Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Neutralization of Zika virus by E protein domain III-Specific human monoclonal antibody

Affiliations

Neutralization of Zika virus by E protein domain III-Specific human monoclonal antibody

Authors

Affiliations

Abstract

Conflict of interest statement

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LinkOut - more resources

Full Text Sources

Other Literature Sources

Medical