doi: 10.1126/science.3353726.
Molecular cloning of human and rat complementary DNA encoding androgen receptors
Affiliations
- PMID: 3353726
- DOI: 10.1126/science.3353726
Item in Clipboard
Molecular cloning of human and rat complementary DNA encoding androgen receptors
Science.
.
Abstract
Complementary DNAs (cDNAs) encoding androgen receptors were obtained from human testis and rat ventral prostate cDNA libraries. The amino acid sequence deduced from the nucleotide sequences of the cDNAs indicated the presence of a cysteine-rich DNA-binding domain that is highly conserved in all steroid receptors. The human cDNA was transcribed and the RNA product was translated in cell-free systems to yield a 76-kilodalton protein. The protein was immunoprecipitable by human autoimmune antibodies to the androgen receptor. The protein bound androgens specifically and with high affinity.
Similar articles
-
Molecular cloning of androgen receptors from divergent species with a polymerase chain reaction technique: complete cDNA sequence of the mouse androgen receptor and isolation of androgen receptor cDNA probes from dog, guinea pig and clawed frog.Biochem Biophys Res Commun. 1990 Sep 14;171(2):697-704. doi: 10.1016/0006-291x(90)91202-4. Biochem Biophys Res Commun. 1990. PMID: 2403358
-
Isolation and characterization of human TR3 receptor: a member of steroid receptor superfamily.J Steroid Biochem. 1989;34(1-6):391-5. doi: 10.1016/0022-4731(89)90114-3. J Steroid Biochem. 1989. PMID: 2626032
-
Specific region in hormone binding domain is essential for hormone binding and trans-activation by human androgen receptor.Mol Endocrinol. 1990 Mar;4(3):417-27. doi: 10.1210/mend-4-3-417. Mol Endocrinol. 1990. PMID: 2342476
-
Cloning and functional expression of the luteinizing hormone receptor complementary deoxyribonucleic acid from the marmoset monkey testis: absence of sequences encoding exon 10 in other species.Endocrinology. 1997 Jun;138(6):2481-90. doi: 10.1210/endo.138.6.5196. Endocrinology. 1997. PMID: 9165039
-
Structural analysis of complementary DNA and amino acid sequences of human and rat androgen receptors.Proc Natl Acad Sci U S A. 1988 Oct;85(19):7211-5. doi: 10.1073/pnas.85.19.7211. Proc Natl Acad Sci U S A. 1988. PMID: 3174628 Free PMC article.
Cited by
-
Role of Sex Steroids in β Cell Function, Growth, and Survival.Trends Endocrinol Metab. 2016 Dec;27(12):844-855. doi: 10.1016/j.tem.2016.08.008. Epub 2016 Sep 15. Trends Endocrinol Metab. 2016. PMID: 27640750 Free PMC article. Review.
-
Caffeic acid phenethyl ester suppresses androgen receptor signaling and stability via inhibition of phosphorylation on Ser81 and Ser213.Cell Commun Signal. 2019 Aug 20;17(1):100. doi: 10.1186/s12964-019-0404-9. Cell Commun Signal. 2019. PMID: 31429764 Free PMC article.
-
Advancing Epidemiology and Genetic Approaches for the Treatment of Spinal and Bulbar Muscular Atrophy: Focus on Prevalence in the Indigenous Population of Western Canada.Genes (Basel). 2023 Aug 17;14(8):1634. doi: 10.3390/genes14081634. Genes (Basel). 2023. PMID: 37628685 Free PMC article. Review.
-
Hormone receptor-related gene polymorphisms and prostate cancer risk in North Indian population.Mol Cell Biochem. 2008 Jul;314(1-2):25-35. doi: 10.1007/s11010-008-9761-1. Epub 2008 May 16. Mol Cell Biochem. 2008. PMID: 18483761
-
From HER2/Neu signal cascade to androgen receptor and its coactivators: a novel pathway by induction of androgen target genes through MAP kinase in prostate cancer cells.Proc Natl Acad Sci U S A. 1999 May 11;96(10):5458-63. doi: 10.1073/pnas.96.10.5458. Proc Natl Acad Sci U S A. 1999. PMID: 10318905 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases
