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. 1988 Jan-Feb;12(1):131-6.
doi: 10.3109/01913128809048481.

A rapid and simple method for electron microscopy of paraffin-embedded tissue

S Widéhn  1 , L G Kindblom
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A rapid and simple method for electron microscopy of paraffin-embedded tissue

S Widéhn et al. Ultrastruct Pathol. 1988 Jan-Feb.

Abstract

A new rapid and simple method for electron microscopy of paraffin-embedded material is described. The paraffin-embedded tissue is deparaffinized in xylene, stained in a 0.01% toluidine blue/absolute ethanol solution, infiltrated in a propylene oxide/resin mixture, and embedded in epoxy resin. This quick method, which excludes rehydration, postosmification, and dehydration, takes about 3 h and produces results equal to previously described more laborious re-embedding techniques.

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