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Comparative Study
. 1988 Apr;163(2):413-9.
doi: 10.1016/0042-6822(88)90282-6.

RNA-dependent RNA polymerase activity in two morphologically different white clover cryptic viruses

G Boccardo  1 , G P Accotto
Affiliations
Comparative Study

RNA-dependent RNA polymerase activity in two morphologically different white clover cryptic viruses

G Boccardo et al. Virology. 1988 Apr.

Abstract

RNA-dependent RNA polymerase activities were detected in purified particles of white clover cryptic viruses 1 and 2. The polymerases of the two viruses had different requirements for optimum activity. Enzyme activity was dependent upon the presence of virus particles, Mg2+, and the four ribonucleoside triphosphates, and was insensitive to actinomycin D, alpha-amanitin, and rifampicin. The labeled reaction products were dsRNAs as indicated by CF 11 column chromatography and by their ionic-strength-dependent sensitivity to hydrolysis by RNase A and resistance to S1 nuclease. The dsRNAs synthesized in vitro had the same electrophoretic mobilities as the corresponding viral templates.

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