A mouse model of short-term, diet-induced fatty liver with abnormal cardiolipin remodeling via downregulated Tafazzin gene expression
- PMID: 33543498
- DOI: 10.1002/jsfa.11144
A mouse model of short-term, diet-induced fatty liver with abnormal cardiolipin remodeling via downregulated Tafazzin gene expression
Abstract
Background: Cardiolipin (CL) helps maintain mitochondrial structure and function. Here we investigated whether a high carbohydrate diet (HCD) fed to mice for a short period (5 days) could modulate the CL level, including that of monolysoCL (MLCL) in the liver.
Results: Total CL in the HCD group was 22% lower than that in the normal chow diet (NCD) group (P < 0.05). The CL72:8 level strikingly decreased by 93% (P < 0.0001), whereas total nascent CLs (CLs other than CL72:8) increased (P < 0.01) in the HCD group. The total MLCL in the HCD group increased by 2.4-fold compared with that in the NCD group (P < 0.05). Tafazzin expression in the HCD group was significantly downregulated compared with that in the NCD group (P < 0.05). A strong positive correlation between nascent CL and total MLCL (r = 0.955, P < 0.0001), and a negative correlation between MLCL and Tafazzin expression (r = -0.593, P = 0.0883) were observed.
Conclusion: A HCD modulated the fatty acid composition of CL and MLCL via Tafazzin in the liver, which could lead to mitochondrial dysfunction. This model may be useful for elucidating the relationship between fatty liver and mitochondrial dysfunction. © 2021 Society of Chemical Industry.
Keywords: high-carbohydrate diet; lipidomics; liquid chromatography mass spectrometry; mitochondria; monolysocardiolipin.
© 2021 Society of Chemical Industry.
References
REFERENCES
-
- Chicco AJ and Sparagna GC, Role of cardiolipin alterations in mitochondrial dysfunction and disease. Am J Physiol Cell Physiol 292:33-44 (2007).
-
- Claypool SM and Koehler CM, The complexity of cardiolipin in health and disease. Trends Biochem Sci 37:32-41 (2012).
-
- Pennington ER, Funaib K, Brown DA and Shaik SR, The role of cardiolipin concentration and acyl chain composition on mitochondrial inner membrane molecular organization and function. Biochim Biophys Acta Mol Cell Biol Lipids 1864:1039-1052 (2019).
-
- Xu Y, Sutachan JJ, Plesken H, Kelley RI and Schlame M, Characterization of lymphoblast mitochondria from patients with Barth syndrome. Lab Invest 85:823-830 (2005).
-
- Song C, Zhang J, Qi S, Liu Z, Zhang X, Zheng Y et al., Cardiolipin remodeling by ALCAT1 links mitochondrial dysfunction to Parkinson's diseases. Aging Cell 18:e12941 (2019).
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- N/A/Grants-in-Aid for Regional R&D Proposal-Based Program from Northern Advancement Center for Science & Technology of Hokkaido
- JP18K07434/Japanese Society for the Promotion of Science KAKENHI Grants
- JP19K07861/Japanese Society for the Promotion of Science KAKENHI Grants
- JP19K2017409/Japanese Society for the Promotion of Science KAKENHI Grants
- Life Science Institute Co., Ltd. and Nissei Bio Co. Ltd
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