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. 2021 Feb 6;37(3):44.
doi: 10.1007/s11274-021-03009-2.

Evidences for antioxidant response and biosorption potential of Bacillus simplex strain 115 against lead

Anissa Chamekh  1 , Oussema Kharbech  1 , Rim Driss-Limam  2 , Cheima Fersi  3 , Mohamed Khouatmeya  2 , Rakia Chouari  4
Affiliations

Evidences for antioxidant response and biosorption potential of Bacillus simplex strain 115 against lead

Anissa Chamekh et al. World J Microbiol Biotechnol. .

Abstract

In this study, we investigated effects of lead on growth response and antioxidant defense protection in a new identified strain isolated from a soil, in the rhizosphere of Sainfoin Hedysarum coronarium L. Different concentrations of lead (0, 0.2, 1.5 and 3 g L-1) added to Bacillus simplex strain 115 cultures surprisingly did not inhibit its growth. However, a resulting oxidative stress as attested by overproduction of H2O2 (+ 6.2 fold) and malondialdehyde (+ 2.3 fold) concomitantly to the enhancement of proteins carbonylation (+ 221%) and lipoxygenase activity (+ 59%) was observed in presence of 3 g L-1 of lead. Intrinsic antioxidant defenses were revealed by the coupled up-regulation of catalase (+ 416%) and superoxide dismutase (+ 4 fold) activities, with a more important Fe-SOD increase in comparison to the other isoforms. Bioaccumulation assays showed both intracellular and extracellular lead accumulation. Biosorption was confirmed as a particularly lead resistance mechanism for Bacillus simplex strain 115 as the metal sequestration in cell wall accounted for 88.5% to 98.5% of the total endogenous metal accumulation. Potentiality of this new isolated microorganism as a biotechnological tool for agricultural soil lead bioremediation was thus proposed.

Keywords: Antioxidant enzymes; Bacillus simplex; Bioaccumulation; Lead tolerance.

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