Flavonoid-rich wheatgrass (Triticum aestivum L.) diet attenuates diabetes by modulating antioxidant genes in streptozotocin-induced diabetic rats

Abstract

Wheatgrass, young germinated shoots of Triticum aestivum L., is proclaimed as antidiabetic nutraceutical by traditional medicines across the world. In this study, the effects of the wheatgrass diet in ameliorating oxidative stress (OS) induced during diabetes were investigated. Total phenolic and flavonoid contents (TPC and TFC) and in vitro antioxidant activity of wheatgrass extract were estimated at different days (5, 7, 9, 11, 13, and 15) after germination. Correlating the TPC and TFC with in vitro antioxidant activity, 9th DAG wheatgrass was found to possess maximum antioxidant potential. UHPLC-MS/MS analysis also revealed the presence of nine flavonoids. For in vivo studies, diabetes was induced by streptozotocin in Wistar rats fed with a high-fat diet. Concomitant administration of 9th-day wheatgrass diet (200 and 400 mg/kg) for 60 days exhibited significant improvements in hyperglycemia, body weight, lipid profile, biochemical indices (AST, ALT, GSH, GPx), and restoration of tissue architectures equivalent to normal rats. Further, qRT-PCR-based expression profiling revealed a significant modulation of major antioxidant marker genes and insulin gene which substantiated that the wheatgrass diet is effective in reducing OS during diabetes. Therefore, flavonoid-rich 9th-day wheatgrass could be used as a functional food to control diabetes. PRACTICAL APPLICATIONS: The present research supported that wheatgrass protects against oxidative stress and therefore could be utilized to ameliorate diabetes. The findings may contribute to the development and formulation of wheatgrass-based functional food or dietary supplement for diabetes by nutraceutical industries.

Keywords: Triticum aestivum; antioxidants; diabetes; flavonoids; gene expression; oxidative stress; qRT-PCR; wheatgrass.