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Observational Study
. 2021 May;99(5):466-471.
doi: 10.1002/cyto.a.24315. Epub 2021 Feb 5.

Monocyte CD169 expression in COVID-19 patients upon intensive care unit admission

Marine Ortillon  1 , Remy Coudereau  1   2 , Martin Cour  3 , Thomas Rimmelé  2   4 , Marine Godignon  1 , Morgane Gossez  1   2 , Hodane Yonis  5 , Laurent Argaud  3 , Anne-Claire Lukaszewicz  2   4 , Fabienne Venet  1   2   6 , Guillaume Monneret  1   2
Affiliations
Observational Study

Monocyte CD169 expression in COVID-19 patients upon intensive care unit admission

Marine Ortillon et al. Cytometry A. 2021 May.

Abstract

During the second surge of COVID-19 in France (fall 2020), we assessed the expression of monocyte CD169 (i.e., Siglec-1, one of the numerous IFN-stimulated genes) upon admission to intensive care units of 45 patients with RT-PCR-confirmed SARS-CoV2 pulmonary infection. Overall, CD169 expression was strongly induced on circulating monocytes of COVID-19 patients compared with healthy donors and patients with bacterial sepsis. Beyond its contribution at the emergency department, CD169 testing may be also helpful for patients' triage at the ICU to rapidly reinforce suspicion of COVID-19 etiology in patients with acute respiratory failure awaiting for PCR results for definitive diagnosis.

Keywords: CD169; COVID-19; monocyte; sepsis.

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Conflict of interest statement

This study was partially supported by Beckman Coulter through donation of the CD169 reagents used in the study. This private company had no role in the study design, or collection and interpretation of the clinical data.

Figures

FIGURE 1
FIGURE 1
Gating strategy (illustrative examples). Monocytes are first gated out from other cells on the basis of on SSC (side‐scatter) / HLA‐DR histogram (blue circle, left histogram) and CD169 is then measured on their surface (monoparametric histogram, right panel) as mean of fluorescence related to the entire monocyte population or as % of positive cells (positivity threshold, red cursor, was based on lack of CD169 expression on neutrophils). In addition, CD169 MFI was also determined on total lymphocyte population gated on SSC / FSC (forward‐scatter) histogram (not shown) in order to provide results CD169 ratio (i.e., mCD169 MFI/lymphocyte CD169 MFI). One illustrative example of each group is depicted (healthy controls, septic shock, COVID‐19) [Color figure can be viewed at wileyonlinelibrary.com]
FIGURE 2
FIGURE 2
Monocyte CD169 expression in COVID‐19 ICU patients. Results are presented as percentages of positive cells (out of total monocyte population, left graph), median of fluorescence intensity in total monocyte population (MFI, middle graph), ratio monocyte CD169 MFI/lymphocyte CD169 MFI (right graph). The blue dashed line represents threshold proposed in Reference . Healthy controls (n = 8) are depicted by empty circles, septic shock patients (n = 2) are depicted by red circles and COVID‐19 ICU patients (n = 45) are depicted by orange circles [Color figure can be viewed at wileyonlinelibrary.com]
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