Micellization of fatty acyl-CoA mixtures and its relevance to the fatty acyl selectivity of acyltransferases

Abstract

The critical micelle concentrations (CMCs) of palmitoyl-CoA/stearoyl-CoA and palmitoyl-CoA/oleoyl-CoA mixtures in 0.050 M KPi, pH 7.4, a buffer used in enzymatic studies, were determined by fluorescence. Mixed micelle solution theory, analogous to the thermodynamic treatment of vapor pressure, was applied to calculate monomer and micelle compositions. The behavior of the palmitoyl-CoA/stearoyl-CoA mixture is ideal, while the palmitoyl-CoA/oleoyl-CoA mixture, although not exhibiting ideal behavior, can be fitted reasonably well by nonideal theory. In both mixtures, selective micellization takes place and, unlike the case of pure fatty acyl-CoAs, above the CMC of the mixtures the concentration of molecules free in solution is strongly dependent upon total concentration. The information derived from the present physical studies becomes important in enzymatic studies with membrane-bound acyltransferases, where selectivity toward various fatty acyl donors, presented as binary mixtures, is frequently observed.