Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 May-Jun;23(3):304-308.
doi: 10.4103/JCD.JCD_425_20. Epub 2020 Dec 4.

Comparative evaluation of pulp tissue dissolution ability of sodium hypochlorite by various activation techniques: An in vitro study

Srividhya Srinivasan  1 , Senthil Kumar Kumarappan  1 , Anupama Ramachandran  1 , Manjiri Nagesh Honap  1 , Sadasiva Kadandale  1 , Sreeram Rayar  1
Affiliations

Comparative evaluation of pulp tissue dissolution ability of sodium hypochlorite by various activation techniques: An in vitro study

Srividhya Srinivasan et al. J Conserv Dent. 2020 May-Jun.

Abstract

Background: The success in endodontic therapy is dependent largely on the absolute elimination of pulp tissue remnants and the maximum reduction of pathologic microbial load present within the root canal system.

Aim: The aim of this in vitro study was to compare and evaluate the ability of pulp tissue dissolution with and without activation of 5.25% sodium hypochlorite (NaOCl).

Materials and methods: Pulp tissue samples collected from intact, noncarious extracted third molars were standardized to 8 mg in each group. These samples were placed in conical Eppendorf tubes of 15 ml with 10 ml 5.25% NaOCl for 4 min and were activated suitably according to the group specifications. All the procedures in this study were carried out at room temperature. The preweighed pulp tissue samples were further weighed again after the dissolution phase, and residual weight loss in each group along with its percentage was obtained. The results were then statistically analyzed using one-way analysis of variance and Tukey post hoc test.

Results: The maximum amount of pulp tissue dissolution was found in the laser-assisted irrigation group, i.e., NaOCl with Er, Cr: YSGG (P < 0.05) and showed a statistically significant result in comparison with other groups.

Conclusion: Within the limitations of the study, laser-assisted irrigation with a radial firing tip (NaOCl activated by Er, Cr: YSGG) was the most effective in dissolving the pulp tissue in comparison with other groups. The ultrasonic activation also showed a considerable amount of pulp tissue dissolution comparable to laser-activated irrigation in this study.

Keywords: Irrigant activation; laser-assisted irrigation; photodynamic therapy; pulp dissolution; sodium hypochlorite.

PubMed Disclaimer

Conflict of interest statement

There are no conflicts of interest.

References

    1. Grossman LI. Solution of pulp tissue by chemical agents. J Endod. 1982;8:3.
    1. Dioguardi M, Gioia GD, Illuzzi G, Laneve E, Cocco A, Troiano G. Endodontic irrigants: Different methods to improve efficacy and related problems. Eur J Dent. 2018;12:459–66. - PMC - PubMed
    1. Boutsioukis C, Lambrianidis T, Kastrinakis E. Irrigant flow within a prepared root canal using various flow rates: A Computational Fluid Dynamics study. Int Endod J. 2009;42:144–55. - PubMed
    1. Sirtes G, Waltimo T, Schaetzle M, Zehnder M. The effects of temperature on sodium hypochlorite short-term stability, pulp dissolution capacity, and antimicrobial efficacy. J Endod. 2005;31:669–71. - PubMed
    1. Mohammadi Z, Shalavi S, Giardino L, Palazzi F, Asgary S. Impact of ultrasonic activation on the effectiveness of sodium hypochlorite: A review. Iran Endod J. 2015;10:216–20. - PMC - PubMed