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. 2020 Nov 18;30(1):159-169.
doi: 10.1007/s10068-020-00843-2. eCollection 2021 Jan.

Optimization of resuscitation-promoting broths for the revival of Vibrio parahaemolyticus from a viable but nonculturable state

Jae-Hyun Yoon  1 , Young-Min Bae  1 , Suyoung Jo  1 , Sung-Kwon Moon  1 , Se-Wook Oh  2 , Sun-Young Lee  1
Affiliations

Optimization of resuscitation-promoting broths for the revival of Vibrio parahaemolyticus from a viable but nonculturable state

Jae-Hyun Yoon et al. Food Sci Biotechnol. .

Abstract

This study was conducted to examine the effect of formulated resuscitation-promoting broths on the revival of viable but nonculturable Vibrio parahaemolyticus induced by cold and starvation stresses. Vibrio parahaemolyticus was incubated in artificial sea water at 4 °C for more than 8 months until this bacterium became undetectable, while retaining its intact cell count of more than 105 CFU/field over time. On day 250, V. parahaemolyticus was collected and enriched in tryptic soy broth supplemented with 3% NaCl, 10,000 U/mg catalase, 2% sodium pyruvate, 20 mM MgSO4, 5 mM EDTA, and a cell-free supernatant taken from V. parahaemolyticus ATCC 17802 in the stationary phase (pH 8). V. parahaemolyticus returned partially to a culturable state with a maximal cell density of 7.91 log CFU/mL in this formulated medium following 7 days of enrichment at 25 °C. In contrast, no V. parahaemolyticus was resuscitated when enriched in alkaline peptone water and tryptic soy broth.

Keywords: Food safety; Pathogen; Resuscitation; Viable but nonculturable; Vibrio parahaemolyticus.

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Conflict of interest statement

Conflict of interestNo conflict of interests was declared.

Figures

Fig. 1
Fig. 1
Changes in (A, C, and E) the colony-forming ability and (B, D, and F) membrane integrity of (A–B) V. parahaemolyticus ATCC 17082, (C–D) V. parahaemolyticus ATCC 33844, and (E–F) V. parahaemolyticus ATCC 27969 inoculated in ASW (pH 6) at 4 °C
See this image and copyright information in PMC

References

    1. Ayrapetyan M, Williams TC, Oliver JD. Interspecific quorum sensing mediates the resuscitation of viable but nonculturable Vibrios. Appl. Environ. Microbiol. 2014;80:2478–2483. doi: 10.1128/AEM.00080-14. - DOI - PMC - PubMed
    1. Bari SMN, Roky MK, Mohiuddin M, Kamruzzaman M, Mekalanos JJ, Faruque SM. Quorum-sensing autoinducers resuscitate dormant Vibrio cholerae in environmental water samples. Proc. Natl. Acad. Sci. USA. 2013;110:9926–9931. doi: 10.1073/pnas.1307697110. - DOI - PMC - PubMed
    1. Cappelier JM, Besnard V, Roche SM, Velge P, Federighi M. Avirulent viable but nonculturable cells of Listeria monocytogenes need the presence of an embryo to be recovered in egg yolk and regain virulence after recovery. Vet. Res. 2007;38:573–583. doi: 10.1051/vetres:2007017. - DOI - PubMed
    1. Chaiyanan S, Chaiyanan SC, Grim C, Maugel T, Huq A, Colwell RR. Ultrastructure of coccoid viable but nonculturable Vibrio cholerae. Environ. Microbiol. 2007;9:393–402. doi: 10.1111/j.1462-2920.2006.01150.x. - DOI - PubMed
    1. Cheng W, Juang FM, Chen JC. The immune response of Taiwan abalone Haliotis diversicolor supertexta and its susceptibility to Vibrio parahaemolyticus at different salinity levels. Fish Shellfish Immunol. 2004;16:295–306. doi: 10.1016/S1050-4648(03)00111-6. - DOI - PubMed