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. 2020 Sep 5;19(2):1205-1214.
doi: 10.1007/s40200-020-00626-w. eCollection 2020 Dec.

Anti-inflammatory and antidiabetic effects of grape-derived stilbene concentrate in the experimental metabolic syndrome

Anatoly Kubyshkin  1 , Alina Shevandova  1 , Vitalina Petrenko  1 , Irina Fomochkina  1 , Leya Sorokina  1 , Alexander Kucherenko  1 , Andrey Gordienko  2 , Natalia Khimich  2 , Evgenia Zyablitskaya  2 , Tatiana Makalish  2 , Leonid Aliev  1 , Natalia Kornienko  1 , Ivan Fomochkin  1
Affiliations

Anti-inflammatory and antidiabetic effects of grape-derived stilbene concentrate in the experimental metabolic syndrome

Anatoly Kubyshkin et al. J Diabetes Metab Disord. .

Abstract

Aims: This study aimed to investigate the carbohydrate and lipid dynamics, associated inflammation markers and the effectiveness of a grape-derived stilbene concentrate (GDSC) treatment in experimental metabolic syndrome (MetS).

Methods: The study was carried out on 40 male 12-weeks of age Wistar rats. The MetS was induced using the fructose model (feeding with 60%-solid fructose diet for 24 weeks). Rats with induced MetS were treated with polyphenolic GDSC, which was obtained by water-alcohol extraction of Vitis vinifera grapevine (Ressfood LLC, Russia).

Results: The experimentally induced MetS development leads to classic MetS signs, including abdominal obesity, hyperglycemia, high lipid levels and heart damage. The expression of glucose transporter type 4 (GLUT4) and peroxisome proliferator-activated receptor-γ (PPAR-γ) had greater dynamics than biochemical measurements. The development of the associated inflammatory reactions was confirmed by the increased level of Toll-like receptor type 4 (TLR4) and C-reactive protein (CRP) compared to control levels. The use of the GDSC had positive dynamics in carbohydrate and lipid levels, inflammatory marker, also prevented associated inflammation and heart damage.

Keywords: Associated inflammation; CRP; GLUT 4; Grape-derived stilbene concentrate; Metabolic syndrome; Molecular markers; PPAR-γ; Polyphenols; TLR 4.

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Conflict of interest statement

Conflict of interestThe authors declare no conflict of interests.

Figures

Fig. 1
Fig. 1
Design of experimental study
Fig. 2
Fig. 2
Abdominal fat. 1: MetS group without treatment; 2: MetS group treated with GDSC beginning the 14th week; 3: MetS group treated with GDSC beginning the 19th week; 4: control group. * - p < 0.001 сompared to control. # - p < 0.001 сompared to MetS group without treatment
Fig. 3
Fig. 3
Glucose levels in MetS after early and late experimental treatment with GDSC. MetS vs Control: ** p < 0.01, *** p < 0.001. MetS+ GDSC since 14 week vs Control: ## p < 0.01, ### p < 0.001. MetS+ GDSC since 19 week vs Control: ++ p < 0.01, MetS+ GDSC since 14 week vs MetS: ••• p < 0.001. MetS+ GDSC since 19 week vs MetS: ⊗ p < 0.05
Fig. 4
Fig. 4
Cholesterol and TGs levels in MS after early and late experimental treatment with GDSC. * - compared to control: ** p < 0.01. # - compared to MetS group without treatment: # p < 0.05, ## p < 0.01, ### p < 0.001
Fig. 5
Fig. 5
40х. А. Fragments of the abdominal aortae from the males in the control group. Paraffin cut. Stained with hematoxylin and eosin. A1 Vessel wall. A2 The elastic framework of the aorta (arrows). В. MS 24th. Fragments of aortae from males with hyperglycemia. Paraffin cut. Stained with hematoxylin and eosin. В1 Loosening of the vessel wall, soaking of the subendothelial intima layer with lipid inclusions (arrow). В2 Damage to the endothelium in the aortic wall (arrow)
Fig. 6
Fig. 6
40х A. Heart fragments from the males in the control group. Paraffin cut. Stained with hematoxylin and eosin. A1. Myocardium of the left ventricle large. A2. Myocardium of the atrium. B. MS 24th. Fragments of the heart from males with hyperglycemia. Paraffin cut. Stained with hematoxylin and eosin. В1. Myocardium of the left ventricle. The phenomena of stasis and perivascular edema with the release of blood cells from the lumen of blood vessels. В2. Endocardium, myocardium. Cleavage of cardiomyocyte bundles (thin arrow), endothelial damage (thick arrow)
Fig. 7
Fig. 7
Scanning electron microscopy of cardiac tissue from rats with MetS (a) and MetS+ GDSC beginning in the 14th week (b). А: Ripped myocardial fibers. B: Saved cardiomyocyte myofibril striation. Abundant myocardial vascularization
Fig. 8
Fig. 8
GLUT4 and PPAR-γ levels in MetS following early and late experimental treatment with GDSC. Compared to control: ** p < 0.01, *** p < 0.001. Compared to MetS group without treatment: # p < 0.05, ## p < 0.01, ### p < 0.001. MetS+GDSC since 14 week vs MetS+GDSC since 19 week: • p < 0.05, •• p < 0.01
Fig. 9
Fig. 9
TLR4 levels and CRP levels in induced MetS rats following early and late experimental treatment with GDSC. Compared to control: ** p < 0.01, *** p < 0.001. Compared to MetS group without treatment: ## p < 0.01, ### p < 0.001. MetS+ GDSC since 14 week vs MetS+ GDSC since 19 week: ••• p < 0.05, ••• p < 0.01
See this image and copyright information in PMC

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