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. 2021 Jan 29;7(1):e06103.
doi: 10.1016/j.heliyon.2021.e06103. eCollection 2021 Jan.

Impact of chemical mutagenesis using ethyl methane sulphonate on tepary bean seedling vigour and adult plant performance

Andries Thangwana  1 , Eastonce T Gwata  1 , Marvelous M Zhou  2
Affiliations

Impact of chemical mutagenesis using ethyl methane sulphonate on tepary bean seedling vigour and adult plant performance

Andries Thangwana et al. Heliyon. .

Abstract

Tepary bean is an important food legume. The genetic improvement of the crop is limited by a narrow genetic base but this genetic base can be broadened through induced mutagenesis. This study was designed to determine the (i) effects of chemical mutagenesis using ethyl methane sulfonate (EMS) on M1 seedlings (ii) the adult plant performance of M2 - M4 generations and (iii) the relationships between growth attributes at the seedling and adult plant stage. Mutagenized seed with varying doses of EMS was germinated at room temperature in order to raise M1 seedlings. There were highly significant (P < 0.01) differences due to dose effects among the seedlings. The highest LD50 (3.37 % EMS v/v) was observed for 'Genotype 3'. Under field conditions, all the three factors influenced the plant performance. The results demonstrated the potential of EMS to induce genotypic variation and desirable agronomic traits in tepary bean.

Keywords: Attributes; Dose; Genotype; Mutagenesis; Seedling.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
M1 seedlings of tepary bean were raised in plastic jars (a) side view and (b) aerial view.
Figure 2
Figure 2
Some seedlings [(a), (c) and (d)] failed to develop roots and remained stunted while others (b) were normal.
Figure 3
Figure 3
The effect of varying doses of ethyl methane sulphonate on (a) percent germination (b) shoot height and (c) secondary root length in three tepary bean genotypes. (G-3 = Genotype 3; G-4 = Genotype 4; G-6 = Genotype 6).
Figure 4
Figure 4
The effect of varying doses of ethyl methane sulphonate on (a) shoot dry weight and (b) root dry weight in three tepary bean genotypes. (G-3 = Genotype 3; G-4 = Genotype 4; G-6 = Genotype 6).
Figure 5
Figure 5
Percent germination and fitted straight lines to estimate the LD50 in three tepary bean genotypes that were treated with varying doses of ethyl methane sulphonate. (G-3 = Genotype 3; G-4 = Genotype 4; G-6 = Genotype 6).
Figure 6
Figure 6
Individual mutant plants segregated for (a) the stay-green trait and earliness and (b) late flowering in the M2 generation.
Figure 7
Figure 7
Individual mutant plants segregated for the number of pods in the M3 generation.
See this image and copyright information in PMC

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