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. 2021 Feb;1(2):e34.
doi: 10.1002/cpz1.34.

Immunopurification of Mitochondria from Arabidopsis

Lilan Luo  1 , Yajun He  1   2 , Jiayang Li  1   2 , Hong Yu  1
Affiliations

Immunopurification of Mitochondria from Arabidopsis

Lilan Luo et al. Curr Protoc. 2021 Feb.

Erratum in

Abstract

Isolation of mitochondria from plant tissues, especially green leaves, is particularly difficult because of chloroplast contamination. Compared to other techniques for purifying plant mitochondria, the immunopurification method has a number of advantages: it rapidly purifies the mitochondria within several minutes, avoids loss of mitochondrial metabolites, eliminates most other organelles (especially the chloroplasts), keeps the isolated mitochondria intact, requires little starting material, and has the potential to purify tissue-specific mitochondria. Here, we describe a rapid immunopurification protocol for mitochondrial isolation that employs a protein specifically targeted to the outer mitochondrial membrane that acts as an immunopurification handle. © 2021 Wiley Periodicals LLC.

Keywords: Arabidopsis; immunopurification; intact mitochondria; mitochondrial isolation; outer mitochondrial membrane.

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References

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