Engineering 3D genome organization

Haifeng Wang^#¹, Mengting Han^#¹, Lei S Qi^{2

3

4}

Affiliations

¹ Department of Bioengineering, Stanford University, Stanford, CA, USA.
² Department of Bioengineering, Stanford University, Stanford, CA, USA. stanley.qi@stanford.edu.
³ Department of Chemical and Systems Biology, Stanford University, Stanford, CA, USA. stanley.qi@stanford.edu.
⁴ ChEM-H Institute, Stanford University, Stanford, CA, USA. stanley.qi@stanford.edu.

^# Contributed equally.

PMID: 33558716
DOI: 10.1038/s41576-020-00325-5

Review

Engineering 3D genome organization

Haifeng Wang et al. Nat Rev Genet. 2021 Jun.

. 2021 Jun;22(6):343-360.

doi: 10.1038/s41576-020-00325-5. Epub 2021 Feb 8.

Authors

Haifeng Wang^#¹, Mengting Han^#¹, Lei S Qi^{2

3

4}

Affiliations

¹ Department of Bioengineering, Stanford University, Stanford, CA, USA.
² Department of Bioengineering, Stanford University, Stanford, CA, USA. stanley.qi@stanford.edu.
³ Department of Chemical and Systems Biology, Stanford University, Stanford, CA, USA. stanley.qi@stanford.edu.
⁴ ChEM-H Institute, Stanford University, Stanford, CA, USA. stanley.qi@stanford.edu.

^# Contributed equally.

PMID: 33558716
DOI: 10.1038/s41576-020-00325-5

Abstract

Cancers and developmental disorders are associated with alterations in the 3D genome architecture in space and time (the fourth dimension). Mammalian 3D genome organization is complex and dynamic and plays an essential role in regulating gene expression and cellular function. To study the causal relationship between genome function and its spatio-temporal organization in the nucleus, new technologies for engineering and manipulating the 3D organization of the genome have been developed. In particular, CRISPR-Cas technologies allow programmable manipulation at specific genomic loci, enabling unparalleled opportunities in this emerging field of 3D genome engineering. We review advances in mammalian 3D genome engineering with a focus on recent manipulative technologies using CRISPR-Cas and related technologies.

PubMed Disclaimer

References

1. Bickmore, W. A. The spatial organization of the human genome. Annu. Rev. Genomics Hum. Genet. 14, 67–84 (2013). - PubMed
1. Clowney, E. J. et al. Nuclear aggregation of olfactory receptor genes governs their monogenic expression. Cell 151, 724–737 (2012). - PubMed - PMC
1. Dekker, J. et al. The 4D nucleome project. Nature 549, 219–226 (2017). - PubMed - PMC
1. Yu, M. & Ren, B. The three-dimensional organization of mammalian genomes. Annu. Rev. Cell Dev. Biol. 33, 265–289 (2017). - PubMed - PMC
1. Zheng, H. & Xie, W. The role of 3D genome organization in development and cell differentiation. Nat. Rev. Mol. Cell Biol. 20, 535–550 (2019). - PubMed

Publication types

Actions
Actions
Actions

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Grants and funding

LinkOut - more resources

Full Text Sources
- Nature Publishing Group
Other Literature Sources
- scite Smart Citations

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Engineering 3D genome organization

Affiliations

Engineering 3D genome organization

Authors

Affiliations

Abstract

References

Publication types

MeSH terms

Grants and funding

LinkOut - more resources

Full Text Sources

Other Literature Sources