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. 2020 Dec;40(6):410-414.
doi: 10.14639/0392-100X-N0490.

Micronucleus, nucleoplasmic bridge and nuclear bud frequencies in patients with laryngeal carcinoma

Ibrahim Yazici  1 , Ozge Caglar  2 , Oguz Guclu  2 , Hayal Cobanoglu  3 , Mahmut Coskun  4 , Münevver Coskun  3 , Aytac Kilic  2 , Fevzi Sefa Dereköy  2
Affiliations

Micronucleus, nucleoplasmic bridge and nuclear bud frequencies in patients with laryngeal carcinoma

Ibrahim Yazici et al. Acta Otorhinolaryngol Ital. 2020 Dec.

Abstract

Micronucleo, ponte nucleoplasmatico e frequenze di gemme nucleari in pazienti con carcinoma laringeo.

Riassunto: Lo scopo dello studio è quello di valutare e confrontare le frequenze relative alla presenza di micronucleo (MN), ponte nucleoplasmatico (NPB) e gemma nucleare (NBUD) in pazienti con carcinoma laringeo e popolazione sana. Lo studio è stato condotto presso un istituto universitario di medicina. Sono stati arruolati 102 volontari, 51 dei quali con carcinoma laringeo e 51 controlli sani. Il metodo di Cytokinesis-Block Micronucleus Assay (CBMN) è stato applicato ai linfociti del sangue periferico dei casi e dei controlli. Abbiamo valutato le frequenze di MN, NPB e NBUD nel paziente con carcinoma laringeo e confrontato i risultati con il gruppo dei controlli. Le frequenze MN, NPB e NBUD dei pazienti con carcinoma laringeo sono risultate significativamente più alte rispetto al gruppo di controllo (P = 0,01, P = 0,004, P = 0,01, rispettivamente). Nello studio, le frequenze MN, NPB e NBUD sono state confrontate in pazienti con e senza esposizione ai pesticidi. I risultati hanno mostrato che la media delle frequenze di NBP, MN e NBUD era significativamente più alta nei pazienti con esposizione ai pesticidi (P = 0,001, P = 0,02 rispettivamente). Il presente studio ha indicato che un aumento delle frequenze di MN, NPB e NBUD nei linfociti periferici umani potrebbe essere utilizzato per prevedere il rischio individuale di sviluppare un carcinoma laringeo. Inoltre, i nostri risultati indicano che l’esposizione ai pesticidi potrebbe aumentare il rischio di carcinoma laringeo.

Keywords: frequency; larynx cancer; micronucleus; nuclear bud; nucleoplasmic bridge.

Plain language summary

The aim of the study is to determine and compare micronucleus (MN), nucleoplasmic bridge (NPB) and nuclear bud (NBUD) frequencies in patients with laryngeal carcinoma and healthy controls. The study was conducted in the School of Medicine of Onsekiz Mart University. A total of 102 volunteers, 51 of whom had laryngeal carcinoma and 51 of whom were healthy control subjects, participated in this study. The Cytokinesis-Block Micronucleus Assay (CBMN) was applied to peripheral blood lymphocytes taken from patients and controls. We evaluated MN, NPBs and NBUDs frequencies in patients with laryngeal carcinoma and compared the results with those in the control group. The frequencies of MN, NPBs and NBUDs of patients with laryngeal carcinoma were found significantly higher than those in the control group (P = 0.01, P = 0.004, P = 0.01, respectively). MN, NPB and NBUD frequencies were also compared in the patients with and without pesticide exposure, and the means of all frequencies was higher in patients with pesticide exposure (P = 0.001, P = 0.02 respectively). The MN, NPBs and NBUDs frequencies of the patients with laryngeal cancer were significantly higher than those of the control group, and pesticide exposure might be a risk factor that increases genomic instability and risk of laryngeal cancer.

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Conflict of interest statement

Conflict of interest

The Authors declare no conflict of interest.

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