Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Jul;54(7):1118-1128.
doi: 10.1111/iej.13495. Epub 2021 Mar 7.

A laboratory study to test the responses of human dental pulp stem cells to extracts from three dental pulp capping biomaterials

A Abou ElReash  1 , H Hamama  2 , M Grawish  3   4 , M Saeed  4 , A M Zaen El-Din  5 , M A Shahin  6 , W Zhenhuan  1 , X Xiaoli  1
Affiliations

A laboratory study to test the responses of human dental pulp stem cells to extracts from three dental pulp capping biomaterials

A Abou ElReash et al. Int Endod J. 2021 Jul.

Abstract

Aim: This laboratory study aimed to investigate the effects of three endodontic biomaterials; MTA-HP, iRoot-BP-Plus and ACTIVA on the proliferation, adhesion and osteogenic differentiation of human Dental Pulp Stem Cells (hDPSCs).

Methodology: The hDPSCs were isolated from the dental pulps of 21 patients scheduled for surgical extraction of their impacted third molars. The MTT assay was used for assessing cellular proliferation. Ninety-six-well plates were used and the experiment was repeated four times under the same condition and the assay was done in triplicate. Four groups were assigned in which the hDPSCs were cultured in complete media only and considered as negative control. Whilst in the 2nd , 3rd and 4th groups, the cells were treated with CM supplemented with 1.5 μl MTA-HP (CM-MTA, iRoot-BP-Plus (CM-BP), and ACTIVA(CM-AC) extracts, respectively. Attachment adhesion and growth morphology of hDPSCs were observed using SEM and the osteogenic differentiation assay was evaluated by Alizarin red stain test (ARS). The data of proliferation and osteogenic differentiation were analysed using two-way ANOVA followed by Tukey's post hoc multiple comparison test. A p-value < 0.05 was considered significant to analyse the differences amongst the means of groups.

Results: Both CM-MTA and CM-BP groups were associated with a significant increase in hDPSC proliferation in comparison with CM-AC and CM groups (p = 0.001). hDPSCs exhibited a greater cellular attachment to iRoot-BP-Plus surfaces followed by MTA-HP, whilst less attachment was observed in the ACTIVA group. Moreover, at day 7 there was a significant difference in formation of mineralizing nodules; CM-BP, CM-MTA and CM-AC groups respectively (p = 0.001). Whilst there was no significance of difference between CM-AC and CM groups (p > 0.05).

Conclusions: In a laboratory setting, ACTIVA, MTA-HP and iRoot-BP-Plus promoted hDPSCs proliferation, mineralization and attachment, which may explain their in-situ success as endodontic biomaterials.

Keywords: bioactive composite; calcium silicate; cytotoxicity; osteogenic differentiation; stem cells.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Abou ElReash A, Hamama H, Abdo W, Wu Q, Zaen El-Din A, Xiaoli X (2019) Biocompatibility of new bioactive resin composite versus calcium silicate cements: an animal study. BMC Oral Health 19, 194.
    1. Ahmed HMA, Luddin N, Kannan TP, Mokhtar KI, Ahmad A (2014) Cell attachment properties of portland cement-based endodontic materials: biological and methodological considerations. Journal of Endodontics 40, 1517-23.
    1. Athanassiadis B, Abbott P, Walsh LJ (2007) The use of calcium hydroxide, antibiotics and biocides as antimicrobial medicaments in endodontics. Australian Dental Journal 52, S64-82.
    1. Awadeen MA, Al-Belasy FA, Ameen LE, Helal ME, Grawish ME (2020) Early therapeutic effect of platelet-rich fibrin combined with allogeneic bone marrow-derived stem cells on rats' critical-sized mandibular defects. World Journal of Stem Cells 12, 55.
    1. Brizuela C, Ormeño A, Cabrera C et al. (2017) Direct pulp capping with calcium hydroxide, mineral trioxide aggregate, and biodentine in permanent young teeth with caries: a randomized clinical trial. Journal of Endodontics 43, 1776-80.

LinkOut - more resources