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. 2021 Sep;131(3):1452-1463.
doi: 10.1111/jam.15037. Epub 2021 Mar 5.

Antifungal effect of volatile organic compounds produced by Streptomyces salmonis PSRDC-09 against anthracnose pathogen Colletotrichum gloeosporioides PSU-03 in postharvest chili fruit

S Boukaew  1 , B Cheirsilp  2 , P Prasertsan  3 , S Yossan  4
Affiliations

Antifungal effect of volatile organic compounds produced by Streptomyces salmonis PSRDC-09 against anthracnose pathogen Colletotrichum gloeosporioides PSU-03 in postharvest chili fruit

S Boukaew et al. J Appl Microbiol. 2021 Sep.

Abstract

Aims: Application of volatile organic compounds (VOCs) from Streptomyces salmonis PSRDC-09 (VOCs PSRDC-09) grown on sterile wheat seeds against chili anthracnose pathogen was investigated in vitro and in vivo.

Methods and results: Among 10 isolates of Colletotrichum species tested, Colletotrichum gloeosporioides PSU-03 was selected as the most aggressive anthracnose pathogenic strain on chili fruit against VOCs produced by Streptomyces species. Among 11 isolates, the strain PSRDC-09 exhibited the highest antifungal activity and was identified as S. salmonis PSRDC-09. The antagonistic mechanism of the VOCs PSRDC-09 on morphological of C. gloeosporioides PSU-03, observed by scanning electron microscope (SEM), revealed the irregular distortions in the fungal hyphae. The effect of inoculum size and spore concentration of S. salmonis PSRDC-09 prepared as a wheat seed inoculum on the suppression of C. gloeosporioides PSU-03 was studied both in vitro and on chili fruit. The optimum inoculum size (45 g 0·31 l-1 ) and spore concentration (107 spores per ml) of the wheat seed culture of S. salmonis PSRDC-09 exhibited the complete suppression (100% inhibition) on C. gloeosporioides PSU-03. The optimum fumigation period of the VOCs PSRDC-09 (45 g 1·38 l-1 ) was found to be 24 h. Based on gas chromatography-mass spectrometry (GC-MS) analysis, 14 major VOCs (produced by the strain PSRDC-09) were detected and l-linalool was the main volatile component.

Conclusions: The results indicated that the VOCs from S. salmonis PSRDC-09 could effectively control the chili anthracnose disease caused by C. gloeosporioides.

Significance and impact of the study: These findings suggest that S. salmonis PSRDC-09 may have the potential to become a promising biofumigant for biocontrol of chili anthracnose disease in the postharvest system.

Keywords: Colletotrichum gloeosporioides; Antifungal effect; Streptomyces salmonis PSRDC-09; biofumigant; volatile organic compounds.

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References

    1. Bailey, J.A. and Jeger, M.J. (1992) Colletotrichum: Biology, Pathology and Control. Wallingford, UK: Commonwealth Mycological Institute.
    1. Boukaew, S., Petlamul, W., Bunkrongcheap, R., Chookaew, T., Kabbua, T., Thippated, A. and Prasertsan, P. (2018) Fumigant activity of volatile compounds of Streptomyces philanthi RM-1-138 and pure chemicals (acetophenone and phenylethyl alcohol) against anthracnose pathogen in postharvest chili fruit. Crop Prot 103, 1-8.
    1. Boukaew, S., Petlamul, W., Phitthayaphinant, P. and Prasertsan, P. (2019) Potential use of Streptomyces mycarofaciens SS-2-243 as a biofumigant to protect maize seeds against two aflatoxin producing fungi. Eur J Plant Pathol 155, 489-503.
    1. Boukaew, S., Plubrukarn, A. and Prasertsan, P. (2013) Effect of volatile substances from Streptomyces philanthi RM-1-138 on growth of Rhizoctonia solani on rice leaf. Biocontrol 58, 471-482.
    1. Boukaew, S. and Prasertsan, P. (2020) Efficacy of volatile compounds from Streptomyces philanthi RL-1-178 as a biofumigant for controlling growth and aflatoxin production of the two aflatoxin-producing fungi on stored soybean seeds. J Appl Microbiol 129, 652-664.

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